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首页> 外文期刊>Clinical Cancer Investigation Journal >Comparing the expression of myoepithelial cell markers CD10 and smooth muscle actin with the estrogen receptor status in the invasive carcinoma breast: An immunohistochemical study
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Comparing the expression of myoepithelial cell markers CD10 and smooth muscle actin with the estrogen receptor status in the invasive carcinoma breast: An immunohistochemical study

机译：侵袭性乳腺癌中肌上皮细胞标志物CD10和平滑肌肌动蛋白的表达与雌激素受体状态的比较：一项免疫组织化学研究

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Background: Breast cancer is the most frequent cancer in females throughout the world. Around 20% of breast carcinomas are estrogen receptor alpha (ER)-negative. Thus, theoretically this negativity could be either the result of down-regulation of ER expression in the tumor cells, or the result of the tumor being derived from cells which normally lack that expression. Normal basal, including myoepithelial cells of the breast is ER-negative. CD10 and smooth muscle actin (SMA) are used as markers for the demonstration of these basal cells. Aims: To compare the expression of positive staining for CD10 and SMA in ER-negative and ER-positive invasive breast carcinomas. Materials and Methods: The study was performed on 40 paraffin-embedded tissues of already diagnosed cases of invasive breast carcinomas with known ER status, i.e., thirty ER-negative and ten ER-positive cases. Expression of CD10 and SMA was demonstrated using avidin-biotin-peroxidase complex (ABC) technique. Tumor was considered to be positive for both markers only when more than 10% of tumor cells were stained positive. Results: Overall, CD10 tumor cell staining was seen in eight, 23.3% (7/30) ER-negative cases and in 10% (1/10) ER-positive cases. Also the staining intensity was considered to be strong. SMA tumor cell staining was seen in only 6.7% (2/30) ER-negative cases and the staining intensity was considered to be moderate. Percentages of positively stained tumor cells varied between 13% to 72% and 23% to 45% for CD10 and SMA, respectively. Conclusion: CD10 is a better marker when compared to SMA, as it is expressed in more number of cases and gives strong positivity in tumor cells. Higher expression of CD10 and SMA is correlated with higher tumor grade and ER negativity.
机译：背景：乳腺癌是全世界女性中最常见的癌症。大约20％的乳腺癌是雌激素受体α（ER）阴性的。因此，从理论上讲，这种否定性可能是肿瘤细胞中ER表达下调的结果，或者是肿瘤源自通常缺乏该表达的细胞的结果。正常的基础，包括乳房的肌上皮细胞是ER阴性的。 CD10和平滑肌肌动蛋白（SMA）用作证明这些基础细胞的标志物。目的：比较CD10和SMA阳性染色在ER阴性和ER阳性浸润性乳腺癌中的表达。材料和方法：本研究是在40例已经诊断为ER状态已知的浸润性乳腺癌病例的石蜡包埋组织中进行的，即30例ER阴性和10例ER阳性。使用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）技术证明了CD10和SMA的表达。仅当超过10％的肿瘤细胞被染色为阳性时，肿瘤才被视为两种标记物均为阳性。结果：总体上，在10例（1/10）ER阳性病例中，有8例23.3％（7/30）ER阴性病例可见CD10肿瘤细胞染色。另外，染色强度被认为是强的。仅6.7％（2/30）ER阴性病例可见SMA肿瘤细胞染色，并且染色强度被认为是中等程度的。对于CD10和SMA，阳性染色的肿瘤细胞百分比分别在13％至72％和23％至45％之间变化。结论：与SMA相比，CD10是更好的标志物，因为它在更多病例中表达，并在肿瘤细胞中具有强阳性。 CD10和SMA的更高表达与更高的肿瘤等级和ER阴性相关。

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