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外文期刊>Clinical epigenetics.
>Newborn genome-wide DNA methylation in association with pregnancy anxiety reveals a potential role for Emphasis Type="Italic"GABBR1/Emphasis
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Newborn genome-wide DNA methylation in association with pregnancy anxiety reveals a potential role for Emphasis Type="Italic"GABBR1/Emphasis
class="Heading">Background id="Par1" class="Para">There is increasing evidence for the role of prenatal stress in shaping offspring DNA methylation and disease susceptibility. In the current study, we aimed to identify genes and pathways associated with pregnancy anxiety using a genome-wide DNA methylation approach. class="Heading">Methods id="Par2" class="Para">We selected 22 versus 23 newborns from our Prenatal Early Life Stress (PELS) cohort, exposed to the lowest or highest degree of maternal pregnancy anxiety, respectively. Cord blood genome-wide DNA methylation was assayed using the HumanMethylation450 BeadChip (HM450, n??=??45) and candidate gene methylation using EpiTYPER (n??=??80). Cortisol levels were measured at 2, 4, and 12??months of age to test infant stress system (re)activity. class="Heading">Results id="Par3" class="Para">Data showed ten differentially methylated regions (DMR) when comparing newborns exposed to low versus high pregnancy anxiety scores. We validated a top DMR in the GABA-B receptor subunit 1 gene (GABBR1) revealing the association with pregnancy anxiety particularly in male newborns (most significant CpG Pearson R??=??0.517, p??=??0.002; average methylation Pearson R??=??0.332, p??=??0.039). Cord blood GABBR1 methylation was associated with infant cortisol levels in response to a routine vaccination at 4??months old. class="Heading">Conclusions id="Par4" class="Para">In conclusion, our results show that pregnancy anxiety is associated with differential DNA methylation patterns in newborns and that our candidate gene GABBR1 is associated with infant hypothalamic-pituitary-adrenal axis response to a stressor. Our findings reveal a potential role for GABBR1 methylation in association with stress and provide grounds for further research.
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机译：class =“ Heading”>背景 id =“ Par1” class =“ Para”>越来越多的证据表明，产前压力在塑造后代DNA甲基化和疾病易感性中的作用。在当前的研究中，我们旨在使用全基因组DNA甲基化方法识别与妊娠焦虑相关的基因和途径。 class =“ Heading”>方法 id =“ Par2”类=“ Para”>我们从产前早期生活压力（PELS）队列中选择了22位与23位新生儿，分别暴露于最低或最高程度的孕产妇焦虑症。使用HumanMethylation450 BeadChip（HM450， n ?? = ?? 45）检测脐血全基因组DNA甲基化，并使用EpiTYPER（ n ?? = ?? 80）。在2、4和12个月大时测量了皮质醇水平，以测试婴儿应激系统的（再）活性。 class =“ Heading”>结果 id =“ Par3”比较暴露于低妊娠焦虑评分和高妊娠焦虑评分的新生儿时，数据显示了十个甲基化差异区域（DMR）。我们验证了GABA-B受体亚基1基因（ GABBR1 ）中的顶级DMR，揭示了其与妊娠焦虑的相关性，特别是在男性新生儿中（最显着的CpG Pearson R ?? = ?? 0.517， p ?? = ?? 0.002;平均甲基化皮尔逊 R ?? = ?? 0.332， p ?? = ?? 0.039）。脐带血 GABBR1 甲基化与婴儿皮质醇水平有关，这是对4个月龄的常规疫苗接种做出的反应。 h3> id =“ Par4” class =“ Para”>最后，我们的结果表明，妊娠焦虑与新生儿的DNA甲基化差异有关，我们的候选基因 GABBR1 与婴儿对压力源的下丘脑-垂体-肾上腺轴反应有关。我们的发现揭示了 GABBR1 甲基化与压力相关的潜在作用，并为进一步研究提供了依据。
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