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首页> 外文期刊>Cogent Environmental Science >Downregulation of liver HSP70 in zebrafish exposed to Jatropha curcas seed cake extract
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Downregulation of liver HSP70 in zebrafish exposed to Jatropha curcas seed cake extract

机译：暴露于麻疯树种子蛋糕提取物的斑马鱼肝脏HSP70的下调

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The use of Jatropha curcas in biodiesel production is one of the emerging alternative sources of renewable energy. However, the extraction process leaves a seed cake waste and the lack of disposal protocols poses serious threat to ecosystems. This study aims to determine the toxicity of the seed cake extract to Danio rerio (zebrafish) through the analysis of heat shock protein 70 expression. The median lethal concentration (LC50) was first determined through an acute toxicity test coupled with probit analysis. For the HSP70 induction test, fishes were subjected to a non-exposed setup and sublethal concentration (50% of LC50) of detoxified, washed, and untreated seed cake. Total RNA from liver samples was isolated and reverse transcribed followed by amplification through polymerase chain reaction (PCR) and gel profiling. Results indicate an LC50 of 0.172% (w/v) or 1.72?g/L. The heat shock induction test showed a significant downregulation of HSP70 expression in fishes exposed to washed and untreated setups. This underexpression may be attributed to phytochemicals such as lectins which have been widely reported to interact with HSP70. Furthermore, mRNA levels in the detoxified setup have no significant difference with the non-exposed setup, possibly indicating a successful inactivation of toxic phytochemicals in the seed cake. Moreover, the present study shows that downregulation of HSP70 is also an indicator of stress, and low HSP70 levels may lead to a decreased resistance of liver cells to stress-inducing agents.
机译：在生物柴油生产中使用麻风树麻疯树是新兴的可再生能源替代来源之一。然而，提取过程留下了种饼废物，缺乏处理协议对生态系统构成了严重威胁。这项研究的目的是通过分析热激蛋白70的表达来确定种子饼提取物对斑马鱼（Danio rerio）（斑马鱼）的毒性。首先通过急性毒性试验和概率分析确定了致死浓度的中位数（LC50）。对于HSP70诱导测试，将鱼置于未暴露的环境中，并进行解毒，洗涤和未处理的种子饼的亚致死浓度（LC50的50％）。从肝脏样品中分离出总RNA，并进行反转录，然后通过聚合酶链反应（PCR）和凝胶分析进行扩增。结果表明LC50为0.172％（w / v）或1.72？g / L。热激诱导试验显示，暴露于洗涤和未处理装置的鱼中HSP70表达显着下调。这种低表达可能归因于植物化学物质，例如凝集素，已被广泛报道与HSP70相互作用。此外，解毒设置中的mRNA水平与未暴露设置中没有显着差异，这可能表明种子饼中的有毒植物化学物质已成功失活。此外，本研究表明，HSP70的下调也是压力的指标，而低的HSP70水平可能导致肝细胞对压力诱导剂的抵抗力降低。

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