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首页> 外文期刊>Cogent Food & Agriculture >Assessment of Ovine Johne’s disease in the Mandya sheep breed in South India using multiple diagnostic tests and bio-typing of Mycobacterium avium subspecies paratuberculosis infection
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Assessment of Ovine Johne’s disease in the Mandya sheep breed in South India using multiple diagnostic tests and bio-typing of Mycobacterium avium subspecies paratuberculosis infection

机译：使用多种诊断测试和鸟分枝杆菌亚种副结核菌感染的生物分型，评估印度南部曼地亚绵羊品种中的绵羊约翰氏病

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Johne’s disease is major infectious disease of domestic livestock in India. Despite low per animal productivity, the country is yet to initiate plan for the survey and control of disease at National scale. A total of 81 clinical samples from Mandya breed of sheep were collected from an organised farm (Livestock Research and Information Centre, Nagmangala) and farmer’s flocks suspected for Johne’s disease on the basis of clinical symptoms (weakness, emaciation, diarrhea and alopecia). Only 10.0% samples from two farms were screened for bio-load of Mycobacterium avium subspecies paratuberculosis (MAP) using multiple tests (microscopy, indigenous ELISA, IS900 PCR and culture). Results showed that 100.0% samples were positive for MAP infection both in microscopy and “Indigenous ELISA”. IS1311 PCR-REA bio-typed 60.0% (feces) and 100.0% (blood and tissue) isolates as “Indian Bison Type”. Typing investigated for the first time that biotype was of Indian origin and present in sheep flocks of South India having wide geographical distribution and broad host range. Study reported high bio-load of MAP in Mandya breed of sheep in Nagamangala, Mandya district and Bilagi taluk of Bagalkot district in South India. Being Johne’s disease a spectral disease, multiple tests were useful for the screening of MAP infection in sheep farms. Study emphasised the need for initiation of short and long term control programs to reduce production losses and sharing of MAP strains by other domestic livestock species and prevent contamination of human food chain and reduce risk to human population.
机译：约翰病是印度家畜的主要传染病。尽管每头动物的生产力低下，该国仍未启动在全国范围内进行疾病调查和控制的计划。从一个有组织的农场（Nagmangala牲畜研究和信息中心）中收集了总共81种来自曼迪亚绵羊的临床样品，并根据临床症状（虚弱，消瘦，腹泻和脱发）怀疑该农场主的鸡群患有约翰氏病。使用多项测试（显微镜，本地ELISA，IS 900 PCR和培养）仅筛选了两个农场的10.0％样品的鸟分枝杆菌亚种（MAP）的生物负荷。结果显示，在显微镜检查和“本地ELISA”检查中，有100.0％的样品对MAP感染呈阳性。 IS 1311 PCR-REA生物分型的60.0％（粪便）和100.0％（血液和组织）分离株为“印第安野牛型”。打字首次调查该生物型起源于印度，并存在于印度南部具有广泛地理分布和寄主范围的羊群中。研究报告称，印度南部的纳甘曼加拉，曼迪亚地区的曼达亚品种和巴加果特地区的比拉吉·塔卢克的羊具有较高的MAP生物负荷。作为约翰尼氏病的一种光谱病，多项测试对筛选绵羊场中的MAP感染很有用。研究强调需要启动短期和长期控制计划，以减少其他家畜物种的生产损失和MAP菌株的共享，并防止人类食物链受到污染并降低对人口的风险。

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