...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cogent Food & Agriculture >Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from seafoods in Lagos Lagoon Nigeria
【24h】

Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from seafoods in Lagos Lagoon Nigeria

机译：在尼日利亚拉各斯泻湖中从海鲜中分离出的副溶血性弧菌的流行和药敏性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study, a total of 90 seafood samples; croaker fish (Pseudotolithus senegalensis ), shrimps (Penaeus notialis ) and blue crab (Callinectes sapidus ) collected from landing sites along the Lagos Lagoon in Nigeria were examined for the prevalence of Vibrio parahaemolyticus using both biochemical and molecular methods. Biochemical identification of the isolates was confirmed by Polymerase Chain Reaction (PCR). The presence of the virulence-associated tdh (thermostable direct haemolysin), trh _(1) (thermostable-related haemolysin) and trh _(2) genes in the V . parahaemolyticus isolates was also detected by the PCR method. PCR products from the V.16S primers were sequenced. Antibiotics susceptibility of the isolates was also determined. About, eight isolates were presumptively identified as V . parahaemolyticus , PCR identified five and none of the isolates were positive for the genes tdh or trh . The five isolates sequenced were identified as different strains of V . parahaemolyticus . V . parahaemolyticus_ RIMD_2210633?=?2MKSHa remained resistant to all antimicrobials tested. However, only V . parahaemolyticus _MP-2_AY911391?=?TBSHy showed strong sensitivity to all the antimicrobials with ampicillin (minimum inhibitory concentration-4?μg/ml). In addition, the other three isolates showed sensitivity for Tetracycline, Ciprofloxacin, Gentamicin and Ceftazidime. Ampicillin resistance in most of the isolates suggests low efficiency of ampicillin in management of V . parahaemolyticus infection.
机译：在这项研究中，总共有90种海鲜样品。利用尼日利亚的拉各斯泻湖沿岸着陆点收集的黄花鱼（Pseudotolithus senegalensis），虾（Penaeus notialis）和蓝蟹（Calliectes sapidus）用于检测副溶血性弧菌的流行情况。生化和分子方法。分离物的生化鉴定通过聚合酶链反应（PCR）得以证实。毒力相关的 tdh（可热固溶的溶血素）， trh _（1）（可热固溶的溶血素）和 trh _（2）基因存在于 V中。还通过PCR方法检测了溶血副溶血菌。对来自V.16S引物的PCR产物进行测序。还确定了分离物的抗生素敏感性。据推测，大约有8个分离株被鉴定为 V。副溶血性，PCR鉴定出五个分离物，且 tdh或 trh基因无阳性。被测序的五个分离株被鉴定为不同的 V株。 parahaemolyticus。 V。 parahaemolyticus_ RIMD_2210633？=？2MKSHa仍然对所有测试的抗生素有抗性。但是，只有 V。副溶血性_MP-2_AY911391？=？TBSHy对氨苄西林的所有抗菌药物（最低抑菌浓度为4？μg/ ml）表现出强烈的敏感性。此外，其他三个分离株对四环素，环丙沙星，庆大霉素和头孢他啶显示敏感性。大多数分离株对氨苄西林的耐药性表明氨苄西林在V的管理中效率较低。 溶血副溶血性感染。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号