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首页> 外文期刊>Clinical, Cosmetic and Investigational Dermatology >Anti-inflammatory and immunomodulatory effects of Aquaphilus dolomiae extract on in vitro models
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Anti-inflammatory and immunomodulatory effects of Aquaphilus dolomiae extract on in vitro models

机译：水生白云石提取物对体外模型的抗炎和免疫调节作用

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Background: Atopic dermatitis (AD) is a common skin disease characterized by recurrent pruritic inflammatory skin lesions resulting from structural and immune defects of the skin barrier. Previous studies have shown the clinical efficacy of Avène thermal spring water in AD, and a new microorganism, Aquaphilus dolomiae was suspected to contribute to these unique properties. The present study evaluated the anti-inflammatory, antipruritic, and immunomodulatory properties of ES0, an original biological extract of A. dolomiae , in immune and inflammatory cell models in order to assess its potential use in the treatment of AD. Materials and methods: An ES0 extract containing periplasmic and membrane proteins, peptides, lipopolysaccharides, and exopolysaccharides was obtained from A. dolomiae . The effects of the extract on pruritus and inflammatory mediators and immune mechanisms were evaluated by using various AD cell models and assays. Results: In a keratinocyte model, ES0 inhibited the expression of the inflammatory mediators, thymic stromal lymphopoietin, interleukin (IL)-18, IL-4R, IL-8, monocyte chemoattractant protein-3, macrophage inflammatory protein-3α, and macrophage-derived chemokine and induced the expression of involucrin, which is involved in skin barrier keratinocyte terminal differentiation. In addition, ES0 inhibited protease-activated receptor-2 activation in HaCaT human keratinocytes stimulated by stratum corneum tryptic enzyme and T helper type (Th) 1, Th2, and Th17 cytokine production in Staphylococcal enterotoxin B–stimulated CD4+ lymphocytes. Lastly, ES0 markedly activated innate immunity through toll-like receptor (TLR) 2, TLR4, and TLR5 activation (in recombinant human embryonic kidney 293 cells) and through antimicrobial peptide induction (psoriasin, human beta-defensin-2, and cathelicidin), mainly through TLR5 activation (in normal human keratinocytes). Conclusion: Overall, these in vitro results confirm the marked regulatory activity of this A. dolomiae extract on inflammatory and immune responses, which may be of value by virtue of its potential as an adjunctive treatment of AD inflammatory and pruritic lesions.
机译：背景：特应性皮炎（AD）是一种常见的皮肤疾病，其特征是皮肤屏障的结构和免疫缺陷导致反复性瘙痒性炎症性皮肤病变。先前的研究表明，雅漾温泉水在AD中的临床功效，并且怀疑一种新的微生物白僵菌（Aquaphilus dolomiae）有助于这些独特的特性。本研究在免疫和炎性细胞模型中评估了白云曲霉原始生物提取物ES0的抗炎，止痒和免疫调节特性，以评估其在AD治疗中的潜在用途。材料和方法：从白僵菌中获得了ES0提取物，其中包含质膜和膜蛋白，肽，脂多糖和胞外多糖。提取物对瘙痒和炎性介质和免疫机制的影响通过使用各种AD细胞模型和测定法进行了评估。结果：在角质形成细胞模型中，ES0抑制炎症介质，胸腺基质淋巴细胞生成素，白介素（IL）-18，IL-4R，IL-8，单核细胞趋化蛋白3，巨噬细胞炎性蛋白3α和巨噬细胞-α的表达。衍生趋化因子并诱导整合素的表达，该整合素参与皮肤屏障角质形成细胞末端分化。此外，ES0抑制了角质层胰蛋白酶和HaocT人角质形成细胞中T辅助型（Th）1，Th2和Th17细胞因子产生的蛋白酶激活的受体2活化，这些细胞因子由葡萄球菌肠毒素B刺激的CD4 +淋巴细胞产生。最后，ES0通过Toll样受体（TLR）2，TLR4和TLR5激活（在重组人胚肾293细胞中）和抗菌肽诱导（psoriasin，人β-defensin-2和cathelicidin）显着激活先天免疫，主要通过TLR5激活（在正常人角质形成细胞中）。结论：总的来说，这些体外结果证实了白果曲霉提取物对炎性和免疫反应的显着调节活性，这可能是由于其作为AD炎性和瘙痒性皮损的辅助治疗的潜力而具有价值。

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