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首页> 外文期刊>Cogent Food & Agriculture >Influence of variety, storage, and simulated gastrointestinal digestion on chemical composition and bioactivity of polysaccharides from sweet cherry and apple tree fruits
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Influence of variety, storage, and simulated gastrointestinal digestion on chemical composition and bioactivity of polysaccharides from sweet cherry and apple tree fruits

机译：品种，储藏和模拟胃肠道消化对甜樱桃和苹果树果实多糖的化学组成和生物活性的影响

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This work examined the effect of extraction regime (hot water vs. simulated in vitro gastrointestinal digestion) and postharvest storage on the chemical composition, molecular weight, and bioactive properties of polysaccharides obtained from sweet cherries (Lapins and Staccato varieties) and apples (Gala and Fuji varieties). The yields of the polysaccharides isolated from cherries and apples ranged from 0.2 to 2.4% on a dry weight fruit basis. All of the isolated polysaccharides contained protein, phenolic compounds, and uronic acid. All polysaccharides contained the sugar monomers: rhamnose, arabinose, xylose, mannose, galactose, and glucose. Also, all of the polysaccharides obtained using the different extraction regimes were shown to possess antioxidant activity as determined with the ferric reducing antioxidant power and the 2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays. Only the polysaccharides isolated from the cherry and apples after simulated in vitro gastrointestinal digestion showed appreciable α-glucosidase inhibition activity, with polysaccharides obtained from Staccato cherries showing nearly 90% of the inhibition of α-glucosidase as achieved with the positive control acarbose. This work shows that bioactive polysaccharides are available and/or can be isolated during digestion which supports the concept that fruit polysaccharides play a role in enhancing human health.
机译：这项工作研究了提取方式（热水与体外胃肠道模拟消化）和采后贮藏对甜樱桃（Lapins和Staccato品种）和苹果多糖的化学组成，分子量和生物活性的影响。 （嘎拉和富士品种）。从樱桃和苹果中分离出的多糖的收率以干重计，为0.2％至2.4％。所有分离的多糖均包含蛋白质，酚类化合物和糖醛酸。所有多糖均包含糖单体：鼠李糖，阿拉伯糖，木糖，甘露糖，半乳糖和葡萄糖。同样，显示出使用不同提取方式获得的所有多糖均具有抗氧化活性，这是通过三价铁还原抗氧化剂能力和2,2-氮杂双（3-乙基-苯并噻唑啉-6-磺酸）（ABTS）自由基清除作用确定的。分析。模拟体外胃肠道消化后，仅从樱桃和苹果中分离出的多糖显示出可观的α-葡萄糖苷酶抑制活性，而从Staccato樱桃获得的多糖显示出阳性对照阿卡波糖对α-葡萄糖苷酶的抑制率接近90％ 。这项工作表明，在消化过程中可以使用和/或可以分离出具有生物活性的多糖，这支持了水果多糖在增强人体健康中起作用的概念。

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