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Determination of distribution constants of antioxidants by electrokinetic chromatography

机译：电动色谱法测定抗氧化剂的分布常数

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Liposome electrokinetic chromatography and micellar electrokinetic chromatography were used for studying the lipophilic properties of natural antioxidants, specifically phenolic acids and flavonoids. The employed negatively charged liposomes were composed of mixtures of 1-palmitoyl-2-oleyl-sn -glycerophosphatidylcholine and 1-palmitoyl-2-oleoyl-sn -phosphatidylserine or 1-palmitoyl-2-oleoyl-sn -glycero-3-phosphatidylglycerol. In micellar electrokinetic chromatography, sodium dodecyl sulphate micelles were used as the pseudostationary phase. The retention factors of the studied compounds were determined at pH 7.4. The corresponding distribution constants were calculated from the experimentally determined retention factors and the phase ratios of the liposome dispersions and the micellar system. The distribution constants between the aqueous phase and the liposomes or micelles were compared with octanol/water partition or distribution constants of the studied compounds, which were predicted using the ACD/Labs Percepta Platform—PhysChem Module. Our results indicate that the correlations between the distribution constants of the two tested liposome systems were much stronger than those between the liposome/micellar systems. The correlations between the n -octanol phase and the liposome phases were similar to that between n -octanol and the micellar phase. Our data shows that electrokinetic chromatography is an efficient method for determining partition coefficients of compounds, but the type of pseudostationary phase has a clear impact on the values.
机译：脂质体电动色谱和胶束电动色谱用于研究天然抗氧化剂，特别是酚酸和类黄酮的亲脂性。所使用的带负电荷的脂质体由1-棕榈酰基-2-油基-sn-甘油磷脂酰胆碱和1-棕榈酰基-2-油酰基-sn-磷脂酰丝氨酸或1-棕榈酰基-2-油酰基-i的混合物组成。 > sn-甘油-3-磷脂酰甘油。在胶束电动色谱中，十二烷基硫酸钠胶束被用作准平稳相。在pH 7.4下确定所研究化合物的保留因子。由实验确定的保留因子以及脂质体分散体和胶束系统的相比计算出相应的分布常数。将水相与脂质体或胶束之间的分布常数与所研究化合物的辛醇/水分配或分布常数进行了比较，这是使用ACD / Labs Percepta Platform-PhysChem模块预测的。我们的结果表明，两个测试脂质体系统的分布常数之间的相关性比脂质体/胶束系统之间的相关性强得多。正辛醇相和脂质体相之间的相关性与正辛醇相和胶束相之间的相关性相似。我们的数据表明，电动色谱是一种确定化合物分配系数的有效方法，但是假平稳相的类型对这些值有明显的影响。

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