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Determination of the antioxidant activity of Limoniastrum feei aqueous extract by chemical and electrochemical methods

机译:化学和电化学方法测定小叶柠檬雪莲水提取物的抗氧化活性

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The total flavonoids (TF), total phenolics, and antioxidant activity of Limoniastrum feei aqueous extract were investigated. The results show that Limoniastrum feei contains 200.28?±?2.75?μg of total phenolic in 1?mg of dry extract, expressed as gallic acid equivalents. The TF represent 54.77?±?3.21?μg/mg, expressed as Quercetin equivalents. The antioxidant activity of extracts has been evaluated by chemical and electrochemical methods. In the reducing power and total antioxidant capacity tests, the antioxidant activity of extracts was expressed as Ascorbic acid equivalents where the aqueous extract has an equivalent capacity of 233.39?±?4.23 and 112.4?±?1.97?μg for 1?mg, respectively. In DPPH? radical trapping test, the IC 50 was equal to 0.58?±?0.03?mg/ml. The cyclic voltammetry of the extract indicates one oxidation irreversible peak at approximately 300–320?mV/(Ag/AgCl). The superoxide scavenging assay of Limoniastrum feei aqueous extract showed an average activity of order 61.46?±?2.51% at 0.5?mg/ml doses.
机译:研究了小白菊水提取物的总类黄酮(TF),总酚类和抗氧化活性。结果表明,在1微克干提取物中,小叶紫草含有200.28±2.75微克的总酚,以没食子酸当量表示。 TF表示为54.77±3.21μg/ mg,以槲皮素当量表示。提取物的抗氧化活性已通过化学和电化学方法进行了评估。在还原能力和总抗氧化能力测试中,提取物的抗氧化活性表示为抗坏血酸当量,其中水提取物的当量容量分别为233.39±4.23和112.4±1.97μg(1 mg)。在DPPH中?自由基捕集试验,IC 50等于0.58≤±0.03μmg/ ml。提取物的循环伏安法表明,在大约300-320?mV /(Ag / AgCl)处有一个氧化不可逆峰。细叶猴毛草水提物的超氧化物清除试验显示,在0.5?mg / ml剂量下,平均活性约为61.46?±?2.51%。

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