...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Comparative and functional genomics >In SilicoIdentification of Functional Protein Interfaces
【24h】

In SilicoIdentification of Functional Protein Interfaces

机译：在计算机上鉴定功能蛋白界面

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Proteins perform many of their biological roles through protein–protein, protein–DNA or protein–ligand interfaces. The identification of the amino acids comprisingthese interfaces often enhances our understanding of the biological function ofthe proteins. Many methods for the detection of functional interfaces have been developed,and large-scale analyses have provided assessments of their accuracy. Amongthem are those that consider the size of the protein interface, its amino acid compositionand its physicochemical and geometrical properties. Other methods to thiseffect use statistical potential functions of pairwise interactions, and evolutionaryinformation. The rationale of the evolutionary approach is that functional and structuralconstraints impose selective pressure; hence, biologically important interfacesoften evolve at a slower pace than do other external regions of the protein. Recently,an algorithm, Rate4Site, and a web-server, ConSurf (http://consurf.tau.ac.il/), forthe identification of functional interfaces based on the evolutionary relations amonghomologous proteins as reflected in phylogenetic trees, were developed in our laboratory.The explicit use of the tree topology and branch lengths makes the methodremarkably accurate and sensitive. Here we demonstrate its potency in the identificationof the functional interfaces of a hypothetical protein, the structure of which wasdetermined as part of the international structural genomics effort. Finally, we proposeto combine complementary procedures, in order to enhance the overall performanceof methods for the identification of functional interfaces in proteins.
机译：蛋白质通过蛋白质-蛋白质，蛋白质-DNA或蛋白质-配体界面执行其许多生物学作用。鉴定包含这些界面的氨基酸通常会增强我们对蛋白质生物学功能的了解。已经开发了许多用于检测功能接口的方法，并且大规模分析已经提供了对其准确性的评估。其中包括考虑蛋白质界面大小，氨基酸组成及其理化和几何性质的蛋白质。实现此目的的其他方法使用成对相互作用的统计潜在函数和进化信息。进化方法的基本原理是功能和结构上的约束施加了选择压力。因此，生物学上重要的界面软化比蛋白质其他外部区域的进化速度慢。最近，开发了一种算法Rate4Site和一个网络服务器ConSurf（http://consurf.tau.ac.il/），用于根据系统进化树中反映的同源蛋白质之间的进化关系来识别功能接口。明确使用树形拓扑和分支长度使该方法非常准确和灵敏。在这里，我们证明了其在鉴定一种假设蛋白质的功能接口方面的潜能，该蛋白质的结构被确定为国际结构基因组学研究的一部分。最后，我们建议结合互补程序，以增强鉴定蛋白质功能界面的方法的整体性能。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号