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Herpesvirus papio 2: Alternative Antigen for Use in Monkey B Virus Diagnostic Assays

机译:疱疹病毒丘疹2:用于猴B病毒诊断测定的替代抗原

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BackgroundandPurpose:SerologictestingforantibodytomonkeyBvirus(BV)inmacaqueseraisproblematicduetothebiohazardousnatureofBVandBVantigens.Herpesviruspapio2(HVP2),aherpesvirusofbaboons,ismorecloselyrelatedgeneticallyandantigenicallytoBVthanishumanherpessimplexvirus1(HSV1).ThepotentialforuseofHVP2relativetoHSV1asanalternativetestantigenfordetectionofanti-BVantibodyinmacaqueserawasassessed.brxmlns="http://pub2web.metastore.ingenta.coms/"/brMethods:StandardELISAformatsweredeveloped,usingBV-,HVP2-,andHSV1-infectedcellextracts.PerformanceoftheHVP2andHSV1testswasassessedrelativetothatoftheBVtest.brxmlns="http://pub2web.metastore.ingenta.coms/"/brResults:UsingtheBVantigenELISA,349serafrom7macaquespeciesweretested,andresultswereclassifiedaspositive(253),negative(94),orsuspect(2).TheELISAusingHVP2antigendetected98.0%ofBV-positivesera(248of253),whereastheHSV1-basedELISAdetectedonly96.0%(243of253).AllthreeELISAsidentifiedthesametwosamplesassuspect,andtheHSV1ELISAidentifiedthreeadditionalBV-positiveseraassuspect.brxmlns="http://pub2web.metastore.ingenta.coms/"/brConclusions:TheHVP2antigen-basedELISAwasequalinsensitivityandspecificitytotheBVantigenbasedELISAandwassuperiortotheHSV1ELISAfordetectionofBV-positivemacaquesera.Inaddition,theHVP2ELISAhasgreaterlaboratorysafety,comparedwithBVantigenuseforELISAtesting.
机译:BackgroundandPurpose:SerologictestingforantibodytomonkeyBvirus(BV)inmacaqueseraisproblematicduetothebiohazardousnatureofBVandBVantigens.Herpesviruspapio2(HVP2),aherpesvirusofbaboons,ismorecloselyrelatedgeneticallyandantigenicallytoBVthanishumanherpessimplexvirus1(HSV1).ThepotentialforuseofHVP2relativetoHSV1asanalternativetestantigenfordetectionofanti-BVantibodyinmacaqueserawasassessed 方法:使用BV-,HVP2-和HSV1感染的细胞提取物开发了标准的ELISA格式。相对于BVtest的性能,评估了HVP2和HSV1测试的性能。 (94),疑似(2)。使用HVP2抗原的ELISA检测到BV阳性血清的98.0%(248of253),而基于HSV1的ELISA检测到只有96.0%(243of253)。所有三种ELISA均确定了相同的两个样品,并且HSV1 ELISA鉴定了三个样品BV阳性血清阳性。

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