Constructing Physical and Genomic Maps forPucciniastriiformisf. sp.tritici, the Wheat Stripe Rust Pathogen, by Comparing Its EST Sequences to the Genomic Sequence ofP. graminisf. sp.tritici, the Wheat Stem Rust Pathogen

JinbiaoMa; XianmingChen; MeinanWang; ZhenshengKang

首页> 外文期刊>Comparative and functional genomics >Constructing Physical and Genomic Maps forPucciniastriiformisf. sp.tritici, the Wheat Stripe Rust Pathogen, by Comparing Its EST Sequences to the Genomic Sequence ofP. graminisf. sp.tritici, the Wheat Stem Rust Pathogen

【24h】

Constructing Physical and Genomic Maps forPucciniastriiformisf. sp.tritici, the Wheat Stripe Rust Pathogen, by Comparing Its EST Sequences to the Genomic Sequence ofP. graminisf. sp.tritici, the Wheat Stem Rust Pathogen

机译：为Pucciniastriiformisf构建物理和基因组图谱。小麦条锈病病原菌sp.tritici，通过将其EST序列与P的基因组序列进行比较。 graminisf。小麦茎锈病病原菌sp.tritici

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The wheat stripe rust fungus,Puccinia striiformisf. sp.tritici(Pst), does not have a known alternate host for sexual reproduction, which makes it impossible to study gene linkages through classic genetic and molecular mapping approaches. In this study, we compared 4,219Pstexpression sequence tags (ESTs) to the genomic sequence ofP. graminisf. sp.tritici(Pgt), the wheat stem rust fungus, using BLAST searches. The percentages of homologous genes varied greatly among differentPstlibraries with 54.51%, 51.21%, and 13.61% for the urediniospore, germinated urediniospore, and haustorial libraries, respectively, with an average of 33.92%. The 1,432Pstgenes with significant homology withPgtsequences were grouped into physical groups corresponding to 237Pgtsupercontigs. The physical relationship was demonstrated by 12 pairs (57%), out of 21 selectedPstgene pairs, through PCR screening of aPstBAC library. The results indicate that thePgtgenome sequence is useful in constructingPstphysical maps.

机译：小麦条纹锈菌，Puccinia striiformisf。 sp.tritici（Pst）没有已知的性繁殖替代宿主，这使得无法通过经典的遗传和分子作图方法研究基因连锁。在这项研究中，我们比较了4,219Ps表达序列标签（EST）与P的基因组序列。 graminisf。使用BLAST搜索的小麦茎锈菌sp.tritici（Pgt）。不同Pslibraries中同源基因的百分比差异很大，其中以乌迪内斯孢子库，发芽的乌迪内斯孢子库和吸排文库分别为54.51％，51.21％和13.61％，平均为33.92％。与Pgts序列具有显着同源性的1,432个Pstgenes被分为对应于237Pgtsupercontig的物理组。通过对aPstBAC文库的PCR筛选，从21对选定的Pstgene对中的12对（57％）证明了这种物理关系。结果表明Pgt基因组序列可用于构建Pstphysical图。

著录项

来源
《Comparative and functional genomics》 |2009年第4期|共13页
作者
JinbiaoMa; XianmingChen; MeinanWang; ZhenshengKang;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类遗传学;
关键词

相似文献

外文文献
中文文献
专利

1. Genomic insights into host adaptation between the wheat stripe rust pathogen (Puccinia striiformis f. sp. tritici) and the barley stripe rust pathogen (Puccinia striiformis f. sp. hordei) [J] . Chongjing Xia, Meinan Wang, Chuntao Yin, BMC Genomics . 2018,第1期

机译：基因组洞察麦芽锈病病原体（PUCCINIA Striformis F.SP.Tritici）与大麦条纹锈病病理学（Puccinia Striformis F.SP。SP。HORDEI）
2. Genome Sequence Resources for the Wheat Stripe Rust Pathogen (Puccinia striiformis f. sp. tritici) and the Barley Stripe Rust Pathogen (Puccinia striiformis f. sp. hordei) [J] . Chongjing Xia, Meinan Wang, Chuntao Yin, Molecular Plant-Microbe Interactions . 2018,第11期

机译：小麦条纹锈病病原体（Puccinia striiformis f。sp。tritici）和大麦条纹锈病病原体（Puccinia striiformis f。hordei）的基因组序列资源。
3. Genome Sequence Resources for the Wheat Stripe Rust Pathogen (Puccinia striiformis f. sp tritici) and the Barley Stripe Rust Pathogen (Puccinia striiformis f. sp hordei) [J] . Xia Chongjing, Wang Meinan, Yin Chuntao, Molecular Plant-Microbe Interactions . 2018,第11期

机译：小麦条纹生锈病原体的基因组序列资源（PUCCINIA Striformis F.SP tritici）和大麦条纹锈病病理原（PUCCINIA Striformis F.SP HORDEI）
4. A physical map of expressed sequence tags and functional genomics in the group 1 chromosomes of wheat Triticum aestivum. [C] . J.H. Peng, H. Zadeh, G. Lazo, International Wheat Genetics Symposium . 2003

机译：小麦小麦染色体中表达序列标签和功能基因组学的物理图。
5. Genomic mapping of leaf rust and stem rust resistance loci in durum wheat and use of RAD-Genotype by Sequencing for the study of population genetics in Puccinia triticina [D] . Aoun, Meriem. 2016

机译：杜兰姆小麦叶片锈病和茎锈抗性基因座的基因组映射及吡锡群群体遗传学研究的用途抗基因型
6. Constructing Physical and Genomic Maps for Puccinia striiformis f. sp. tritici the Wheat Stripe Rust Pathogen by Comparing Its EST Sequences to the Genomic Sequence of P. graminis f. sp. tritici the Wheat Stem Rust Pathogen [O] . Jinbiao Ma, Xianming Chen, Meinan Wang, 2009

机译：构建小麦条锈菌的物理和基因组图谱。 sp。小麦条纹锈病病原体通过将其EST序列与gras。graminis f。的基因组序列进行比较sp。小麦茎锈病
7. Constructing Physical and Genomic Maps for Puccinia striiformis f. sp. tritici, the Wheat Stripe Rust Pathogen, by Comparing Its EST Sequences to the Genomic Sequence of P. graminis f. sp. tritici, the Wheat Stem Rust Pathogen [O] . Ma, Jinbiao, Chen, Xianming, Wang, Meinan, 2009

机译：构建小麦条锈菌的物理和基因组图谱。 sp。小麦条纹锈病病原体小麦，通过将其EST序列与gras。graminis f。的基因组序列进行比较sp。小麦茎锈病

Constructing Physical and Genomic Maps forPucciniastriiformisf. sp.tritici, the Wheat Stripe Rust Pathogen, by Comparing Its EST Sequences to the Genomic Sequence ofP. graminisf. sp.tritici, the Wheat Stem Rust Pathogen

摘要

著录项

相似文献

相关主题

期刊订阅