A New Apparatus and Surgical Technique for the Dual Perfusion of Human Tumor Xenografts in Situ in Nude Rats

Dauchy Robert T; Dauchy Erin M; Mao Lulu; Belancio Victoria P; Hill Steven M; Blask David E

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A New Apparatus and Surgical Technique for the Dual Perfusion of Human Tumor Xenografts in Situ in Nude Rats

机译：裸鼠原位双重灌注人肿瘤异种移植物的新器械和手术技术

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Wepresentanewperfusionsystemandsurgicaltechniqueforsimultaneousperfusionof2tissue-isolatedhumancancerxenograftsinnuderatsbyusingdonorbloodthatpreservesacontinuousflow.Adult,athymicnuderats(Hsd:RH-Foxn1suprnu/sup)wereimplantedwithHeLahumancervicalorHT29colonadenocarcinomasandgrownastissue-isolatedxenografts.Whentumorsreachedanestimatedweightof5to6g,ratswerepreparedforperfusionwithdonorbloodandarteriovenousmeasurements.Thesurgicalprocedurerequiredapproximately20mintocompleteforeachtumor,andtumorswereperfusedforaperiodof150min.Resultsshowedthattumorvenousbloodflow,glucoseuptake,lacticacidrelease,Osub2/subuptakeandCOsub2/subproduction,uptakeoftotalfattyacidandlinoleicacidandconversiontothemitogen13-HODE,cAMPlevels,andactivationofseveralmarkerkinaseswereallwellwithinthenormalphysiologic,metabolic,andsignalingparameterscharacteristicofindividuallyperfusedxenografts.Thisnewperfusionsystemandtechniquereducedproceduretimebymorethan50%.Thesefindingsdemonstratethat2humantumorscanbeperfusedsimultaneouslyinsituorexvivobyusingeitherrodentorhumanbloodandsuggestthatthesystemmayalsobeadaptedforuseinthedualperfusionofotherorgans.Advantagesofthisdualperfusiontechniqueincludedecreasedanesthesiatime,decreasedsurgicalmanipulation,andincreasedefficiency,therebypotentiallyreducingthenumbersoflaboratoryanimalsrequiredforscientificinvestigations.

机译：Wepresentanewperfusionsystemandsurgicaltechniqueforsimultaneousperfusionof2tissue-isolatedhumancancerxenograftsinnuderatsbyusingdonorbloodthatpreservesacontinuousflow.Adult，athymicnuderats（HSD：RH-FOXN1 ^{RNU ）wereimplantedwithHeLahumancervicalorHT29colonadenocarcinomasandgrownastissue-isolatedxenografts.Whentumorsreachedanestimatedweightof5to6g，ratswerepreparedforperfusionwithdonorbloodandarteriovenousmeasurements.Thesurgicalprocedurerequiredapproximately20mintocompleteforeachtumor，andtumorswereperfusedforaperiodof150min.Resultsshowedthattumorvenousbloodflow，glucoseuptake，lacticacidrelease，O- <子> 2 uptakeandCO最终，该_{2 的生产量和技术水平可以确定，这是随着时间的流逝，这两种新的灌注系统所能产生的50％的_{2 产量，摄取的总脂肪酸和亚油酸以及转化为促分裂原13-HODE，cAMP水平和几种标记激酶的活化在正常的生理，代谢和信号参数特征下都得到了证实。通过使用啮齿动物或人类的血液同时融合在原位或体内，并建议该系统也可用于其他器官的二次灌注。这种双重灌注技术的优点包括降低麻醉时间，减少手术操作，提高效率，降低效率。}}}

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《Comparative Medicine》 |2012年第2期|共页
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Dauchy Robert T; Dauchy Erin M; Mao Lulu; Belancio Victoria P; Hill Steven M; Blask David E;
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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1. A New Apparatus and Surgical Technique for the Dual Perfusion of Human Tumor Xenografts in Situ in Nude Rats [J] . Dauchy Robert T., Dauchy Erin M., Mao Lulu, Comparative Medicine . 2012,第2期

机译：裸鼠原位双重灌注人肿瘤异种移植物的新器械和手术技术
2. Rapid in situ hybridization technique for detecting malignant mouse cell contamination in human xenograft tissue from nude mice and in vitro cultures from such xenografts. [J] . Gao J, Tombal B, Isaacs JT The Prostate . 1999,第1期

机译：快速原位杂交技术可检测裸鼠和异种移植物的体外培养物中人异种移植组织中的恶性小鼠细胞污染。
3. Co-implantation of Tumor and Extensive Surrounding Tissue Improved the Establishment Rate of Surgical Specimens of Human-Patient Cancer in Nude Mice: Toward the Goal of Universal Individualized Cancer Therapy [J] . TAKUYA MURATA, CHIHIRO HOZUMI, YUKIHIKO HIROSHIMA, In vivo. . 2020,第6期

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4. Study on the Antitumor Effect of Sonodynamic Therapy on Nude Mice Bearing Intracranial Glioblastoma Xenografts [C] . Yongpeng Huang, Zhaoke Pi, Xin Chen, IEEE International Ultrasonics Symposium . 2018

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5. Antiproliferative effects of isoflavonoids on prostate cancer cells and antitumorigenic effects in xenograft nude mice. [D] . Samedi, Von Graeger. 2001

机译：异黄酮类化合物对前列腺癌细胞的抗增殖作用和异种移植裸鼠的抗肿瘤作用。
6. A New Apparatus and Surgical Technique for the Dual Perfusion of Human Tumor Xenografts in Situ in Nude Rats [O] . Robert T Dauchy, Erin M Dauchy, Lulu Mao, 2012

机译：裸鼠原位双重灌注人肿瘤异种移植物的新器械和手术技术
7. Therapeutic effect against human xenograft tumors in nude mice by the third generation microtubule stabilizing epothilones [O] . Chou, Ting-Chao, Zhang, Xiuguo, Zhong, Zi-Yang, 2008

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A New Apparatus and Surgical Technique for the Dual Perfusion of Human Tumor Xenografts in Situ in Nude Rats

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