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Microbial-Enhanced Heavy Oil Recovery under Laboratory Conditions by Bacillus firmus BG4 and Bacillus halodurans BG5 Isolated from Heavy Oil Fields

机译:实验室条件下分离自重油田的坚固芽孢杆菌BG4和嗜碱性芽孢杆菌BG5在实验室条件下的微生物强化重油回收

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Microbial Enhanced Oil Recovery (MEOR) is one of the tertiary recovery methods. The high viscosity and low flow characteristics of heavy oil makes it difficult for the extraction from oil reservoirs. Many spore-forming bacteria were isolated from Oman oil fields, which can biotransform heavy crude oil by changing its viscosity by converting heavier components into lighter ones. Two of the isolates, Bacillus firmus BG4 and Bacillus halodurans BG5, which showed maximum growth in higher concentrations of heavy crude oil were selected for the study. Gas chromatography analysis of the heavy crude oil treated with the isolates for nine days showed 81.4% biotransformation for B. firmus and 81.9% for B. halodurans. In both cases, it was found that the aromatic components in the heavy crude oil were utilized by the isolates, converting them to aliphatic species. Core flooding experiments conducted at 50 ?°C, mimicking reservoir conditions to prove the efficiency of the isolates in MEOR, resulted in 10.4% and 7.7% for B. firmus and B. halodurans, respectively, after the nine-day shut-in period. These investigations demonstrated the potential of B. firmus BG4 and B. halodurans BG5 as an environmentally attractive approach for heavy oil recovery.
机译:微生物强化采油(MEOR)是三次采油方法之一。重油的高粘度和低流量特性使其难以从储油层中提取。从阿曼油田分离出许多形成孢子的细菌,这些细菌可以通过将较重的组分转化为较轻的组分来改变其粘度,从而对重质原油进行生物转化。选择了两个分离株,即在较高浓度的重质原油中表现出最大生长的牢固芽孢杆菌BG4和嗜碱性芽孢杆菌BG5。气相色谱分析分离物处理了9天的重质原油显示,坚硬芽孢杆菌的生物转化率为81.4%,卤代芽孢杆菌的生物转化率为81.9%。在这两种情况下,都发现重质原油中的芳族成分被分离物利用,将它们转化为脂肪族物质。在九天的关闭期后,在50°C下进行的岩心驱油实验模拟了储层条件,以证明分离物在MEOR中的有效性,导致了坚强芽孢杆菌和嗜盐芽孢杆菌分别获得10.4%和7.7%的收获。 。这些研究证明了牢固的芽孢杆菌BG4和卤代芽孢杆菌BG5作为一种环境有吸引力的重油回收方法。

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