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Production of a Biosurfactant by Cunninghamella echinulata Using Renewable Substrates and Its Applications in Enhanced Oil Spill Recovery

机译:利用可更新的底物生产棘孢枯草生物表面活性剂及其在溢油回收中的应用

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This study aimed to evaluate the production of a surfactant by Cunninghamella echinulata, using agro-industrial residues, corn steep liquor (CSL), and soybean oil waste (SOW). The study had a factorial design, using as a variable response to the reduction of surface tension. C. echinulata was able to produce biosurfactant in assay, CSL (8.82%) and SOW (2%). The results showed that the biosurfactant was successfully produced by C. echinulata and had attractive properties, such as a low surface tension (31.7 mN/m), a yield of 5.18 g/L at 120 h of cultivation, and an anionic profile. It also achieved a reduction in surface tension stability in a wide range of pH values, temperatures, and salinity values. The biosurfactant produced by C. echinulata showed an absence of toxicity to Artemia salina. The influence of the biosurfactant on the viscosity of engine oil, burnt engine oil, diesel, soybean oil post-frying, canola oil, and water was investigated. The results reveal a mechanism for the decrease of the viscosity using hydrophobic substrates and the new biosurfactant solution at 1.5% of the (CMC). This enables the formulation of a low-cost culture medium alternative, based on corn steep liquor and the reuse of soybean oil after frying to produce a biosurfactant. Additionally, performance of the biosurfactant isolated from C. echinulata showed an excellent ability to remove spilled oil, such as diesel (98.7%) and kerosene (92.3%) from marine sand.
机译:这项研究旨在评估利用农业工业残留物,玉米浆(CSL)和大豆油废料(SOW)来生产Cunninghamella echinulata的表面活性剂。该研究采用析因设计,将其作为对表面张力降低的变量响应。 echinulata能够在测定中产生生物表面活性剂,CSL(8.82%)和SOW(2%)。结果表明,该生物表面活性剂是由棘孢梭菌成功制备的,具有吸引人的特性,如低表面张力(31.7 mN / m),在120 h培养下产量为5.18 g / L和阴离子型。在较宽的pH值,温度和盐度值范围内,也降低了表面张力的稳定性。 C. echinulata生产的生物表面活性剂对卤虫卤虫没有毒性。研究了生物表面活性剂对机油,燃烧机油,柴油,大豆油后油炸,低芥酸菜子油和水的粘度的影响。结果揭示了使用疏水性底物和1.5%(CMC)的新生物表面活性剂溶液降低粘度的机理。这使得能够基于玉米浸泡液和油炸后再生产大豆油以生产生物表面活性剂的基础上,配制低成本的培养基替代品。另外,从棘孢梭菌分离的生物表面活性剂的性能显示出优异的能力,能够从海砂中去除溢油,例如柴油(98.7%)和煤油(92.3%)。

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