Embryo Transfer Rederivation of C.B-17/Icr-Prkdcscid Mice Experimentally Infected with Mouse Parvovirus 1

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Embryo Transfer Rederivation of C.B-17/Icr-Prkdcscid Mice Experimentally Infected with Mouse Parvovirus 1

机译：实验感染小鼠细小病毒1的C.B-17 / Icr-Prkdcscid小鼠的胚胎移植重新活化

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WedeterminedwhetherembryosderivedfromC.B-17/Icr-Prkdcsupscid/sup(SCID)miceinfectedwithmouseparvovirus(MPV)1bandmatedtoMPV-na?veB6C3F1micewouldtransmitvirustona?verecipientfemalemiceandrederivedprogeny.ViralDNAwasdetectedbyquantitativePCR(qPCR)inlymphoidtissues,gonad,sperm,andfecesofallMPV1b-inoculatedSCIDmice.ViralDNAwasdetectedin1of16aliquotsofembryosfrominfectedmaleSCIDmiceandin12of18aliquotsofembryosfrominfectedfemaleSCIDmice.AllrecipientfemalemiceimplantedwithembryosfrominfectedSCIDmalemiceandtheirprogenywerenegativebyserologyandqPCR.Incontrast,3of5recipientfemalemiceimplantedwithembryosfrominfectedSCIDfemalemiceand14of15progenymicefromtheserecipientswereseropositivebymultiplexfluorescentimmunoassay(MFI)forMPVcapsidantigen(rVP2).AllofthesemicewerenegativebyMFIforparvovirusnonstructuralproteinantigen(rNS1)andbyqPCR,withtheexceptionof1recipientfemalemousethatdisplayedweakrNS1seroreactivityandlowlevelsofMPVDNAinlymphoidtissues.SeroreactivitytorVP2declinedovertimeinallprogenymicefrominfectedSCIDfemalemiceuntilallwereseronegativeby20wkofage,consistentwithmaternalantibodytransfer.GiventhatthehighlevelsofMPVcontaminationdetectedinourexperimentallyinfectedSCIDmiceareunlikelyinnaturallyinfectedimmunocompetentmice,thesedataindicatethatembryotransferrederivationiseffectivefortheeradicationofMPVfrominfectedcolonies.

机译：WedeterminedwhetherembryosderivedfromC.B-17 / ICR-PRKDC ^{SCID （SCID）miceinfectedwithmouseparvovirus（MPV）1bandmatedtoMPV-NA？veB6C3F1micewouldtransmitvirustona？verecipientfemalemiceandrederivedprogeny.ViralDNAwasdetectedbyquantitativePCR（qPCR的）inlymphoidtissues，性腺，精子，andfecesofallMPV1b-inoculatedSCIDmice.ViralDNAwasdetectedin1of16aliquotsofembryosfrominfectedmaleSCIDmiceandin12of18aliquotsofembryosfrominfectedfemaleSCIDmice.AllrecipientfemalemiceimplantedwithembryosfrominfectedSCIDmalemiceandtheirprogenywerenegativebyserologyandqPCR。 Incontrast，3of5recipientfemalemiceimplantedwithembryosfrominfectedSCIDfemalemiceand14of15progenymicefromtheserecipientswereseropositivebymultiplexfluorescentimmunoassay（MFI）forMPVcapsidantigen（rVP2）.AllofthesemicewerenegativebyMFIforparvovirusnonstructuralproteinantigen（rNS1）andbyqPCR，withtheexceptionof1recipientfemalemousethatdisplayedweakrNS1seroreactivityandlowlevelsofMPVDNAinlymphoidtissues.SeroreactivitytorVP2declinedovertimeinallprogenymicefr最小化的SCID雌性小鼠直到20周后才阴性，与母体抗体转移一致。鉴于在实验性感染的SCID小鼠中检测到高水平的MPV污染很可能在自然感染的免疫力小鼠中很可能被检测到，这些数据表明从复制的胚胎中分离出有效的MPV。}

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《Comparative Medicine》 |2008年第4期|共页
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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1. Embryo transfer rederivation of C.B-17/Icr-Prkdc(scid) mice experimentally infected with mouse parvovirus 1. [J] . Besselsen DG, Romero-Aleshire MJ, Munger SJ, Comparative Medicine . 2008,第4期

机译：实验感染鼠细小病毒1的C.B-17 / Icr-Prkdc（scid）小鼠的胚胎转移重新活化。
2. Temporal transmission studies of mouse parvovirus 1 in BALB/c and C.B-17/Icr-Prkdcscid mice. [J] . Besselsen D G, Becker M D, Henderson K S, Comparative Medicine . 2007,第1期

机译：小鼠细小病毒1在BALB / c和C.B-17 / Icr-Prkdcscid小鼠中的时间传播研究。
3. Strain- and Age-Associated Variation in Viral Persistence and Antibody Response to Mouse Parvovirus 1 in Experimentally Infected Mice [J] . Filipovska-Naumovska Emilija, Thompson Martin J., Hopwood Deborah, Journal of the American Association for Laboratory Animal Science . 2010,第4期

机译：在实验感染小鼠中病毒持久性和对小鼠细小病毒1的抗体应答的应变和年龄相关的变异。
4. Study on the Effect of Baicalin and Ligustrazine on Early Stage Embryos in Vitro Culture and the Frozen-Thawed Embryos Transfer in Mice [C] . The First Academic Conference of Asian Society of Traditional Veterinary Medicine(首届亚洲兽医传统医学会学术研讨会)论文集 . 2006

机译：黄ical苷和川gust嗪对小鼠体外培养早期胚胎及冻融胚胎移植的影响
5. Experimental study of the effects of green tea on improving the outcomes of BALB/c mice infected with Leishmania mexicana [D] . Avila, Alejandra 2009

机译：绿茶改善墨西哥利什曼原虫感染的BALB / c小鼠预后的实验研究
6. Embryo Transfer Rederivation of C.B-17/Icr-Prkdcscid Mice Experimentally Infected with Mouse Parvovirus 1 [O] . David G Besselsen, Melissa J Romero-Aleshire, Stephanie J Munger, 2008

机译：实验感染小鼠细小病毒1的C.B-17 / Icr-Prkdcscid小鼠的胚胎移植重新活化
7. Comparison of polymerase chain reaction and culture for detection of Borrelia burgdorferi in naturally infected Peromyscus leucopus and experimentally infected C.B-17 scid/scid mice [O] . E K Hofmeister, R B Markham, J E Childs, 1992

机译：聚合酶链反应和培养物检测天然感染的细胞菌白细胞和实验感染的C.B-17 SCID / SCID小鼠的培养基培养物

Embryo Transfer Rederivation of C.B-17/Icr-Prkdcscid Mice Experimentally Infected with Mouse Parvovirus 1

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