Mutational Insertion of a ROSA26&#8211;EGFP Transgene Leads to Defects in Spermiogenesis and Male Infertility in Mice

Walters Eric M; Bauer Beth A; Franklin Craig L; Evans Tim J; Bryda Elizabeth C; Riley Lela K; Critser John K

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Mutational Insertion of a ROSA26–EGFP Transgene Leads to Defects in Spermiogenesis and Male Infertility in Mice

机译：ROSA26＆＃8211; EGFP转基因的突变插入导致小鼠精子发生和男性不育的缺陷。

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Pronuclearinjectionhasbeenasuccessfulstrategyforgeneratinggeneticallyengineeredmousemodelstobetterunderstandthefunctionalityofgenes.Acharacteristicofpronuclearinjectionisthatrandomintegrationofthetransgeneintothegenomecandisturbafunctionalgeneandresultinaphenotypeunrelatedtothetransgeneitself.Inthisstudy,wehavecharacterizedamousemodelcontaininganinsertionalmutationthat,inthehomozygousstate,severelyaffectsspermatogenesisascharacterizedbylackofspermmotilityandacrosomalaplasia.Whereashomozygousfemalemicehadnormalfertility,malemicehomozygousfortheinsertionalmutationwereunabletoproducepupsbynaturalmatingwitheitherhomozygousorwild-typefemalemice.Nofertilizedembryoswereproducedbymatingstohomozygousmalemice,andnospermwerepresentinthereproductivetractofmatedfemalemice.Spermatozoaisolatedfromhomozygousmalemiceexhibitedheadandmidpiecedefects,butnomajordefectsintheprincipalpieceofthesesperm.HistologicexaminationandimmunohistochemicalstainingofthetestesrevealedvacuolardegenerationofSertolicellsandlossofstructuralseminiferoustubuleintegrityandorganization,indicatingthatspermatogenesisisseverelyaffectedinthismousemodel.Althoughthemalesarealwaysinfertile,theseverityofthehistologicandspermmorphologicdefectsappearedtobeage-related.

机译：Pronuclearinjectionhasbeenasuccessfulstrategyforgeneratinggeneticallyengineeredmousemodelstobetterunderstandthefunctionalityofgenes.Acharacteristicofpronuclearinjectionisthatrandomintegrationofthetransgeneintothegenomecandisturbafunctionalgeneandresultinaphenotypeunrelatedtothetransgeneitself.Inthisstudy，wehavecharacterizedamousemodelcontaininganinsertionalmutationthat，inthehomozygousstate，severelyaffectsspermatogenesisascharacterizedbylackofspermmotilityandacrosomalaplasia.Whereashomozygousfemalemicehadnormalfertility，malemicehomozygousfortheinsertionalmutationwereunabletoproducepupsbynaturalmatingwitheitherhomozygousorwild-typefemalemice.Nofertilizedembryoswereproducedbymatingstohomozygousmalemice，andnospermwerepresentinthereproductivetractofmatedfemalemice.Spermatozoaisolatedfromhomozygousmalemiceexhibitedheadandmidpiecedefects，butnomajordefectsintheprincipalpieceofthesesperm.HistologicexaminationandimmunohistochemicalstainingofthetestesrevealedvacuolardegenerationofSertolicellsandl结构性有孔小管的完整性和组织性，表明这种小鼠模型的生精能力受到严重影响。尽管雄性不育，但组织学和精子形态学缺陷的严重性似乎与衰老有关。

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《Comparative Medicine》 |2009年第6期|共页
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Walters Eric M; Bauer Beth A; Franklin Craig L; Evans Tim J; Bryda Elizabeth C; Riley Lela K; Critser John K;
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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1. Mutational Insertion of a ROSA26-EGFP Transgene Leads to Defects in Spermiogenesis and Male Infertility in Mice. [J] . Walters Eric M., Bauer Beth A., Franklin Craig L., Comparative Medicine . 2009,第6期

机译：ROSA26-EGFP转基因的突变插入导致小鼠精子发生和男性不育的缺陷。
2. Ggnbp2 -Null Mutation in Mice Leads to Male Infertility due to a Defect at the Spermiogenesis Stage [J] . Lingyun Liu, Yan He, Kaimin Guo, American Journal of Pathology: Official Publication of the American Association of Pathologists . 2017,第11期

机译：ggnbp2-null突变在小鼠中导致男性不孕症由于精氨酸阶段的缺陷
3. A nonsense mutation in Ccdc62 gene is responsible for spermiogenesis defects and male infertility in repro29/repro29 mice [J] . Li Yuchi, Li Cailing, Lin Shouren, Biology of Reproduction: Offical Journal of the Society for the Study of Reproduction . 2017,第3期

机译：CCDC62基因中的无意义突变是对精灵发生的缺陷和雄性不孕症，在Repro29 / Repro29小鼠中
4. (00588)Comparative Effects of Salinomycin and Meso-2,3-Dimercaptosuccinic Acid (DMSA)on Lead-Induced Impairment of Reproductive Function in Male Mice [C] . Pavlova E., Kamenova K., Gluhcheva Y. International Conference on Environmental Science and Technology . 2019

机译：（00588）盐霉素和Meso-2,3-二巯基琥珀酸（DMSA）对雄性小鼠生殖功能损伤的对比作用
5. Characterization of infertile male mice with ampullar innervation defects resulting from targeting of the Evx1 gene. [D] . Wieckowski, Peter J. 2007

机译：不育雄性小鼠的特征，该小鼠具有因靶向Evx1基因而引起的壶腹神经缺损。
6. Ggnbp2-Null Mutation in Mice Leads to Male Infertility due to a Defect at the Spermiogenesis Stage [O] . Lingyun Liu, Yan He, Kaimin Guo, 2017

机译：小鼠的Ggnbp2-Null突变由于在精子形成阶段的缺陷而导致男性不育。
7. A nonsense mutation in Ccdc62 gene is responsible for spermiogenesis defects and male infertility in repro29/repro29 mice † [O] . Yuchi Li, Cailing Li, Shouren Lin, 2017

机译：CCDC62基因中的无意义突变是对精灵发生的缺陷和雄性不孕症，在Repro29 / Repro29小鼠中†

Mutational Insertion of a ROSA26&#8211;EGFP Transgene Leads to Defects in Spermiogenesis and Male Infertility in Mice

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Mutational Insertion of a ROSA26–EGFP Transgene Leads to Defects in Spermiogenesis and Male Infertility in Mice