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首页> 外文期刊>Comparative and functional genomics >Comparison of the Proteomes of Three Yeast Wild Type Strains: CEN.PK2, FY1679 and W303
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Comparison of the Proteomes of Three Yeast Wild Type Strains: CEN.PK2, FY1679 and W303

机译：三种酵母野生型菌株CEN.PK2，FY1679和W303的蛋白质组比较

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摘要

Yeast deletion strains created during gene function analysis projects very often showdrastic phenotypic differences depending on the genetic background used. These resultsindicate the existence of important molecular differences between the CEN.PK2, FY1679and W303 wild type strains. To characterise these differences we have compared theprotein expression levels between CEN.PK2, FY1679 and W303 strains using twodimensionalgel electrophoresis and identified selected proteins by mass spectrometricanalysis. We have found that FY1679 and W303 strains are more similar to each otherthan to the CEN.PK2 strain. This study identifies 62 proteins that are differentiallyexpressed between the strains and provides a valuable source of data for the interpretationof yeast mutant phenotypes observed in CEN.PK2, FY1679 and W303 strains.
机译：在基因功能分析项目中产生的酵母缺失菌株经常会出现严重的表型差异，具体取决于所使用的遗传背景。这些结果表明CEN.PK2，FY1679和W303野生型菌株之间存在重要的分子差异。为了表征这些差异，我们使用二维凝胶电泳比较了CEN.PK2，FY1679和W303菌株之间的蛋白质表达水平，并通过质谱分析鉴定了选定的蛋白质。我们已经发现，FY1679和W303菌株比CEN.PK2菌株彼此更相似。这项研究确定了菌株之间差异表达的62种蛋白质，并为解释CEN.PK2，FY1679和W303菌株中观察到的酵母突变表型提供了有价值的数据来源。

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