Characterization of the Interaction of Escherichia coli Heat-Stable Enterotoxin (STa) with its Intestinal Putative Receptor in Various Age Groups of Mice, Using Flow Cytometry and Binding Assays

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Characterization of the Interaction of Escherichia coli Heat-Stable Enterotoxin (STa) with its Intestinal Putative Receptor in Various Age Groups of Mice, Using Flow Cytometry and Binding Assays

机译：使用流式细胞术和结合分析表征大肠杆菌热稳定肠毒素（STa）及其肠道假定受体在不同年龄组中的相互作用

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BackgroundandPurpose:EnterotoxigenicEscherichiacoliheat-stableenterotoxin(STa)isamajorcauseofdiarrheainyounganimals.Age-dependentvariationinthedensityandaffinityofthemouseenterocytereceptorsspecificforSTawasinvestigated.brxmlns="http://pub2web.metastore.ingenta.coms/"/brMethods:Fouragegroups(2-day-,1-and2-week-,and2-month-old)ofSwissWebstermicewerestudied(8to10mice/group).FlowcytometryandradiolabeledSTa(125I-STa)assayswereusedasreliablequantitativemeasuresforcharacterizationofSTa-enterocytereceptorinteraction.brxmlns="http://pub2web.metastore.ingenta.coms/"/brResultsandConclusions:InteractionofSTawithitsputativereceptorwasstrongerforenterocytesof2day-oldmice.Scatchardanalysisof125I-STa-receptorinteractionsuggestedthatSTa-receptorsexistathighernumbersonenterocytesfrom2-day-old(7.2nmol/mg)thanolder(0.30,0.36,and0.40nmol/mgfor1-week-,2week-,and2-month-oldmice,respectively).Additionally,receptorsfrom2-day-oldmicehadgreateraffinityforSTa(Ksubxmlns="http://pub2web.metastore.ingenta.coms/"d/sub=75nM)thandidreceptorsfromoldermice(Ksubxmlns="http://pub2web.metastore.ingenta.coms/"d/sub=125,1,430,and1,111nMfor1-week-,2-week-,and2-month-oldmice,respectively).DensityofSTareceptorsonenterocytesandtheiraffinitytoSTamaydetermineextentofbindingandseverityofthesecretoryresponse,andmayexplainthehighsusceptibilityofnewbornanimalsandhumaninfantstoSTa-mediateddiarrhea.

机译：背景和目的：产肠毒素的大肠杆菌毒素肠毒素（STa）主要是幼小的动物腹泻的原因。特定于STawas的小鼠肠细胞受体的密度和亲和力的年龄依赖性变化。d=75nM）来自老小鼠的胆碱受体（K d = 125、1,430和1 ，分别在1周，2周和2个月大的小鼠中为111nM。）ST受体细胞的密度和对Samay的亲和力决定了分泌反应的结合程度和严重性，并可能解释了新生动物和人类婴儿对STa介导的腹泻的高敏感性。

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《Comparative Medicine》 |1999年第3期|共页
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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1. Characterization of the interaction of Escherichia coli heat-stable enterotoxin (STa) with its intestinal putative receptor in various age groups of mice, using flow cytometry and binding assays [J] . Ahmad M. Al-Majali, J. Paul Robinson, Elikplimi K. Asem Laboratory Animal Science . 1999,第3期

机译：使用流式细胞仪和结合测定法表征不同年龄组小鼠中大肠杆菌热稳定肠毒素（STa）及其肠推定受体的相互作用
2. Use of flow cytometry to measure the interaction between Escherichia coli heat-stable enterotoxin and its intestinal receptor in mice. [J] . Al Majali-AM, Robinson JP, Asem EK, Journal of Immunological Methods . 1999,第1a2期

机译：使用流式细胞仪测量小鼠大肠杆菌中热稳定肠毒素与其肠受体之间的相互作用。
3. Interaction of Escherichia coli heat-stable enterotoxin (STa) with its putative receptor on the intestinal tract of newborn kids. [J] . Al-Majali AM, Ababneh MM, Shorman M, FEMS immunology and medical microbiology . 2007,第1期

机译：大肠杆菌热稳定肠毒素（STa）及其推定受体在新生婴儿肠道中的相互作用。
4. Nanoparticulated Heat-Stable(STa)and Heat-Labile B Subunit(LTB)Recombinant Toxin Improves Vaccine Protection Against Enterotoxigenic Escherichia coli Challenge in Mouse [C] . Deng Guangcun, Zeng Jin, Jian Minjie, 16th International congress on animal hygiene : animal hygiene, health and welfare as corner stones of sustainable animal production . 2013

机译：纳米颗粒热稳定（STa）和不耐热B亚基（LTB）重组毒素可提高疫苗抵抗小鼠肠毒素性大肠杆菌的挑战
5. Enterotoxigenic Escherichia coli promotes its adherence to intestinal epithelial cells through heat-labile enterotoxin-dependent and independent mechanisms. [D] . Johnson, Amber M. 2008

机译：产肠毒素的大肠杆菌通过不耐热的肠毒素依赖性和非依赖性机制来促进其对肠上皮细胞的粘附。
6. Solubilization and partial characterization of the intestinal receptor for Escherichia coli heat-stable enterotoxin. [O] . L A Dreyfus, D C Robertson 1984

机译：大肠埃希氏菌热稳定肠毒素的肠道受体的增溶和部分表征。
7. Solubilization and partial characterization of the intestinal receptor for Escherichia coli heat-stable enterotoxin [O] . L A Dreyfus, D C Robertson 1984

机译：大肠杆菌热稳定肠毒素的肠道受体的溶解和部分表征
8. Enteroaggregative Escherichia coli Heat-Stable Enterotoxin 1 Represents AnotherSubfamily of E. coli Heat-Stable Toxin. (Reannouncement with New Availability Information) [R] . Savarino, S. J., Fasano, A., Watson, J., 1993

机译：肠聚集性大肠杆菌热稳定肠毒素1代表另一个大肠杆菌热稳定毒素家族。（重新公布新的可用性信息）

Characterization of the Interaction of Escherichia coli Heat-Stable Enterotoxin (STa) with its Intestinal Putative Receptor in Various Age Groups of Mice, Using Flow Cytometry and Binding Assays

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