Monoclonal Antibodies Reactive with Dendritic Cells of Mongolian Gerbils

Sato Hiroshi; Chisty Moinuddin; Nargis Mafhuza; Inaba Takashi; Yagisawa Makoto; Kamiya Haruo

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Monoclonal Antibodies Reactive with Dendritic Cells of Mongolian Gerbils

机译：与蒙古沙鼠的树突状细胞反应的单克隆抗体

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Mongoliangerbils(Merionesunguiculatus)serveasanvaluablemodelanimalforseveralinfectiousdiseasesofmedicalandveterinaryimportance.ReagentsavailableforcharacterizationoftheimmuneresponseofMongoliangerbilsarestrictlylimited.Wedescribethreenovelmurinemonoclonalantibodies(mAbs)todendriticcellsofMongoliangerbils.TheseincludeHUSM-M.g.11ofIgG2bisotype,HUSM-M.g.20ofIgG2aisotype,andHUSM-M.g.30ofIgG1isotype.AllofthesemAbshadanidenticalprofileofimmunohistochemicalreactionswithvarioustissuestakenfromimmune-naiveMongoliangerbils,andwereintensivelyexpressedondendriticcells,includingepidermalLangerhanscells,B-cellfollicles,andthethymicreticulum.PositivereactionsoftheepidermisandintestinalmucosawiththesemAbswereinducedbycutaneousorintestinalinfectionswithparasites.Competitiveenzymelinkedimmunosorbentassayandimmunoblotanalysis(westernblotting)indicatedthatallofthesemAbsrecognizeanidenticalpeptideepitopeonamoleculewithapproximatemolecularmassof29kDa.ThesedatasuggestthatthemAbsrecognizemajorhistocompatibilitycomplexclass-IImoleculesofgerbils.UseofdescribedmAbswouldfacilitatecharacterizationofimmuneresponsesaswellasinvestigationsonhostresponsestoinfectionsofmedicalandveterinaryimportance,usingthegerbilmodel.

机译：Mongoliangerbils（Merionesunguiculatus）serveasanvaluablemodelanimalforseveralinfectiousdiseasesofmedicalandveterinaryimportance.ReagentsavailableforcharacterizationoftheimmuneresponseofMongoliangerbilsarestrictlylimited.Wedescribethreenovelmurinemonoclonalantibodies（MABS）todendriticcellsofMongoliangerbils.TheseincludeHUSM-Mg11ofIgG2bisotype，HUSM-Mg20ofIgG2aisotype，andHUSM-Mg30ofIgG1isotype.AllofthesemAbshadanidenticalprofileofimmunohistochemicalreactionswithvarioustissuestakenfromimmune-naiveMongoliangerbils，andwereintensivelyexpressedondendriticcells，includingepidermalLangerhanscells，B-cellfollicles，andthethymicreticulum.PositivereactionsoftheepidermisandintestinalmucosawiththesemAbswereinducedbycutaneousorintestinalinfectionswithparasites.Competitiveenzymelinkedimmunosorbentassayandimmunoblotanalysis（westernblotting）indicatedthatallofthesemAbsrecognizeanidenticalpeptideepitopeonamoleculewithapproximatemolecularmassof29kDa这些数据表明mAb可以识别主要的组织相容性鼠类复合体II类分子。使用上述单克隆抗体将有助于利用沙鼠模型来表征免疫应答的特征以及研究其宿主对医疗和兽医感染的反应。

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《Comparative Medicine》 |2001年第3期|共页
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Sato Hiroshi; Chisty Moinuddin; Nargis Mafhuza; Inaba Takashi; Yagisawa Makoto; Kamiya Haruo;
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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专利

1. Human blood dendritic cell-like B cells isolated by the 5G9 monoclonal antibody reactive with a novel 220-kDa antigen. [J] . Zhong RK, Donnenberg AD, Zhang HF, The Journal of Immunology: Official Journal of the American Association of Immunologists . 1999,第3期

机译：通过与新型220 kDa抗原反应的5G9单克隆抗体分离的人血树突状细胞样B细胞。
2. Anti-Thy-1 Monoclonal Antibody-Induced Glomerulonephritis in Mongolian Gerbils [J] . Comparative Medicine . 2000,第6期

机译：抗Thy-1单克隆抗体诱导的蒙古沙鼠肾小球肾炎
3. The anti-rat Thy-1.1 monoclonal antibody R2-12C5 recognizing rat, mouse, hamster, and Mongolian gerbil Thy-1 antigen had no effect on IL-2 induced rat thymocyte proliferation. [J] . Kinebuchi M, Matsuura A, Uede T, Transplantation Proceedings . 1999,第3期

机译：识别大鼠，小鼠，仓鼠和蒙古沙土鼠Thy-1抗原的抗大鼠Thy-1.1单克隆抗体R2-12C5对IL-2诱导的大鼠胸腺细胞增殖没有影响。
4. Efficient Acquisition of Antigen-Specific Human Monoclonal Antibody by Using Peripheral Blood Mononuclear Cells Immunized In Vitro Efficient Cloning of Human Monoclonal Antibody Gene [C] . Shinei Matsumoto, Makiko Yamashita, Yoshinori Katakura, Annual Meeting of the Japanese Association for Animal Cell Technology . 2009

机译：通过使用在体外克隆人单克隆抗体基因的体外克隆的外周血单核细胞高效采集抗原特异性人单克隆抗体
5. Targeted delivery of antigens to follicular dendritic cells via a monoclonal antibody specific for complement receptor 2 on marginal zone B cells: Dynamics of delivery and applications towards vaccine development. [D] . Whipple, Emily Christine. 2006

机译：通过对边缘B区细胞的补体受体2特异的单克隆抗体，将抗原靶向递送至滤泡树突状细胞：动力学和疫苗开发应用。
6. Dendritic Morphology of Caudal Periaqueductal Gray Projecting Retinal Ganglion Cells in Mongolian Gerbil (Meriones unguiculatus) [O] . Chaoran Ren, Mingliang Pu, Qi Cui, -1

机译：蒙古沙鼠尾侧心前导水管灰色投射视网膜神经节细胞的树突形态学（Meriones unguiculatus）
7. Flow cytometric assessment of the reactivity of a panel of monoclonal antibodies (mAb) against two populations of human dendritic cells (DC) [O] . Nunez Rafael, Filgueira Luis 2001

机译：流式细胞仪评估一组单克隆抗体（mAb）对两种人类树突状细胞（DC）的反应性

Monoclonal Antibodies Reactive with Dendritic Cells of Mongolian Gerbils

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