Exposure of Preimplantation Embryos to Insulin Alters Expression of Imprinted Genes

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Exposure of Preimplantation Embryos to Insulin Alters Expression of Imprinted Genes

机译：胰岛素植入前胚胎的暴露改变了印迹基因的表达

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Insulinpromotesearlyembryonicdevelopment,butwhetherthisactionaffectspostimplantationfetaldevelopmentandalterstheexpressionofimprintedgenesremaintobedetermined.ThisstudyanalyzedtheexpressionandmethylationlevelsofthegrowthrelatedimprintedgenesH19andinsulin-likegrowthfactor2(Igf2)infetusesexposedtoinsulinbeforeimplantation.Wecultured2-cellembryosineither0or0.25#59157;g/mlinsulinuntiltheblastocyststageandthentransferredthemintopseudopregnantrecipientmice.Thenumberofembryosdevelopingtoblastocystsafterinsulinexposurewas16.4%higherthanthatofthecontrol,andthebirthbodyweightoftheinsulin-exposedgroupwas17.8%higherthanthatofthecontrolgroup.Real-timereversetranscription#8211;polymerasechainreactionanalysisrevealedthatexposureofpreimplantationembryostoinsulinincreasedthemRNAexpressionofbothIgf2andH19inembryonicday(E)14fetuses.BisulfitegenomicsequencingdemonstratedthatthemethylationleveloftheH19#8211;Igf2imprintcontrolregionwas19.3%lowerininsulin-exposedE14fetusesthanincontrols.Thepresentstudyindicatesthatinsulinexposureduringthepreimplantationstagealterstheexpressionofimprintedgenesandaffectsfetaldevelopment.

机译：Insulinpromotesearlyembryonicdevelopment，butwhetherthisactionaffectspostimplantationfetaldevelopmentandalterstheexpressionofimprintedgenesremaintobedetermined.ThisstudyanalyzedtheexpressionandmethylationlevelsofthegrowthrelatedimprintedgenesH19andinsulin-likegrowthfactor2（IGF2）infetusesexposedtoinsulinbeforeimplantation.Wecultured2-cellembryosineither0or0.25＃59157克/ mlinsulinuntiltheblastocyststageandthentransferredthemintopseudopregnantrecipientmice.Thenumberofembryosdevelopingtoblastocystsafterinsulinexposurewas16.4％higherthanthatofthecontrol，andthebirthbodyweightoftheinsulin-exposedgroupwas17.8％higherthanthatofthecontrolgroup.Real-timereversetranscription＃8211; polymerasechainreactionanalysisrevealedthatexposureofpreimplantationembryostoinsulinincreasedthemRNAexpressionofbothIgf2andH19inembryonicday（E）14fetuses。亚硫酸氢盐基因组测序证实，H19＃8211; Igf2印迹控制区域的甲基化水平比对照中胰岛素暴露的E14胎儿低19.3％。接受测试的胰岛素在植入前阶段会改变印迹的表达并影响胎儿的发育。

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《Comparative Medicine》 |2007年第5期|共页
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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1. Exposure of preimplantation embryos to insulin alters expression of imprinted genes [J] . Shao WJ, Tao LY, Xie JY, Comparative Medicine . 2007,第5期

机译：植入前胚胎暴露于胰岛素会改变印迹基因的表达
2. Effects of in vitro oocyte maturation and embryo culture on the expression of glucose transporters, glucose metabolism and insulin signaling genes in rhesus monkey oocytes and preimplantation embryos [J] . Ping Zheng1 Rita Vassena1 and Keith E. Latham123 Molecular Human Reproduction . 2007,第6期

机译：体外卵母细胞成熟和胚胎培养对恒河猴卵母细胞和植入前胚胎葡萄糖转运蛋白表达，葡萄糖代谢和胰岛素信号转导基因的影响
3. Effects of in vitro oocyte maturation and embryo culture on the expression of glucose transporters, glucose metabolism and insulin signaling genes in rhesus monkey oocytes and preimplantation embryos [J] . Zheng P., Vassena R., Latham K.E. Molecular human reproduction. . 2007,第6期

机译：体外卵母细胞成熟和胚胎培养对恒河猴卵母细胞和着床前胚胎葡萄糖转运蛋白表达，葡萄糖代谢和胰岛素信号转导基因的影响
4. The expression of reference genes affecting preimplantation mouse embryo death in the DDK syndrome [C] . Gendi Song, Baoguo Chai, Chunli Li, 2011 International Conference on Remote Sensing, Environment and Transportation Engineering . 2011

机译：影响DDK综合征植入前小鼠胚胎死亡的参考基因的表达
5. Individual bull variations on sperm acrosome reaction, sperm -zona binding, in-vitro embryo production, and preimplantation embryo apoptosis and gene expression [D] . Giritharan, Gnanaratnam 2004

机译：公牛对精子顶体反应，精子-zona结合，体外胚胎产生以及植入前胚胎凋亡和基因表达的变异
6. Early prenatal alcohol exposure alters imprinted gene expression in placenta and embryo in a mouse model [O] . Heidi Marjonen, Mia Toivonen, Laura Lahti, 2012

机译：早期产前酒精暴露会改变小鼠模型中胎盘和胚胎中的印迹基因表达
7. Igf2r and Igf2 gene expression in androgenetic, gynogenetic, and parthenogenetic preimplantation mouse embryos: absence of regulation by genomic imprinting. [O] . K E Latham, A S Doherty, C D Scott, 1994

机译：IGF2R和IGF2基因表达在雄激素，洋母和单性前胚胎小鼠胚胎中：通过基因组印记的缺乏调节。

Exposure of Preimplantation Embryos to Insulin Alters Expression of Imprinted Genes

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