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首页> 外文期刊>Comparative and functional genomics >Transcriptome Analysis of Rainbow Trout (Oncorhynchus mykiss) Eggs Subjected to the High Hydrostatic Pressure Treatment
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Transcriptome Analysis of Rainbow Trout (Oncorhynchus mykiss) Eggs Subjected to the High Hydrostatic Pressure Treatment

机译：高压水处理虹鳟卵的转录组分析

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High hydrostatic pressure (HHP) causes depolymerization of the spindle microtubules. HHP applied to fish eggs prevents extrusion of the second polar body and inhibits the first cell cleavage, and it is used to produce triploids and diploid gynogenetic and androgenetic individuals. HHP has been also found to affect biomolecules including nucleic acids, and it may be presumed that HHP administered to the rainbow trout (Oncorhynchus mykiss) eggs disturbs cytoplasmic maternal RNA indispensable for the early embryogenesis. To verify this assumption, quality and quantity of RNA extracted from the rainbow trout eggs subjected to the high hydrostatic pressure shock were analyzed. Provided results exhibited that maternal transcriptome was resistant to a three-minute exposure to 65.5 MPa of HHP treatment. Some trend showing increase of the RNA integrity was observed in the HHP-treated eggs; however, the difference was not statistically significant. Alterations in the expression profiles in the rainbow trout eggs subjected to HHP were also negligible. Greater differences in the maternal gene expression were observed between eggs from different clutches than between HHP-treated and untreated eggs from the same clutch. It may be assumed that exposure to HHP shock was too short to modify significantly maternal transcripts in the rainbow trout eggs.
机译：高静水压（HHP）导致纺锤体微管解聚。应用于鱼卵的HHP可以防止第二极体的挤出并抑制第一细胞的分裂，它可用于生产三倍体和二倍体雌雄同体和雄激素个体。还已经发现HHP会影响包括核酸在内的生物分子，并且可以推测对虹鳟卵（Oncorhynchus mykiss）卵施用的HHP会干扰早期胚胎发生必不可少的细胞质母体RNA。为了验证该假设，分析了经受高静水压力冲击的虹鳟鱼卵中提取的RNA的质量和数量。提供的结果表明，母本转录组对暴露于65.5 MPa的HHP处理过程中的三分钟有抵抗力。在HHP处理的鸡蛋中观察到一些显示RNA完整性增加的趋势;但是，差异在统计上并不显着。接受HHP处理的虹鳟鱼卵中表达谱的变化也可以忽略不计。观察到来自不同离合器的卵之间的母本基因表达差异大于来自相同离合器的HHP处理和未处理的卵之间的差异。可以假设暴露于HHP休克太短而不能显着改变虹鳟鱼卵中的母体转录本。

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