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首页> 外文期刊>Comparative and functional genomics >Transcriptomic Changes in Broiler Chicken Hypothalamus during Growth and Development
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Transcriptomic Changes in Broiler Chicken Hypothalamus during Growth and Development

机译：肉鸡下丘脑生长发育过程中的转录组学变化

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The hypothalamus plays an overarching role that is reflected in the physiological processes observed in the entire organism. The hypothalamus regulates selected metabolic processes and activities of the autonomic nervous system. The avian hypothalamus due to the structural complexity is not well described and has a slightly different function than the mammalian hypothalamus that is the subject of numerous studies. The present study evaluated activities of hypothalamic genes in fast-growing chickens during development (at the 1st day and 3rd and 6th weeks after hatching). The hypothalamic transcriptomes for 3- and 6-week-old cockerels were analysed using an RNA sequencing method in next-generation sequencing (NGS) technology. The differentially expressed gene analysis was conducted using DESeq2 software. In younger 22-day-old cockerels, 389 genes showed higher expression (fold change > 1.5) than that in 45-day-old birds. These genes played a role in several biological processes because they encoded proteins involved in integrin signalling, regulation of hormone levels, camera-type eye development, and blood vessel development. Moreover, surprisingly in the hypothalamus of 3-week-old cockerels, transcripts were identified for proteins involved in both anorexigenic (POMC, NMU) and orexigenic (PMCH, ALDH1A1, LPL, and GHRH) pathways. The RNA-seq results were confirmed by qPCR methods. In summary, the intensive growth of 3-week-old chickens was reflected in hypothalamic activities because the genes associated with the somatotropin axis and regulation of satiety centre showed increased expression.
机译：下丘脑起着至关重要的作用，这在整个生物体中观察到的生理过程中得到了反映。下丘脑调节自主神经系统的选定代谢过程和活动。禽下丘脑由于结构复杂而没有得到很好的描述，其功能与哺乳动物下丘脑的功能略有不同，哺乳动物下丘脑是众多研究的主题。本研究评估了发育过程中（孵化后第1天，第3周和第6周）下丘脑中下丘脑基因的活性。使用下一代测序（NGS）技术中的RNA测序方法分析了3周龄和6周龄公鸡的下丘脑转录组。使用DESeq2软件进行差异表达基因分析。在22日龄的幼小公鸡中，有389个基因的表达（倍数> 1.5）比45日龄的高。这些基因在几个生物学过程中发挥了作用，因为它们编码参与整联蛋白信号传导，激素水平调节，相机型眼睛发育和血管发育的蛋白质。此外，令人惊讶的是，在三周大的公鸡的下丘脑中，发现了与厌食症（POMC，NMU）和致呕性（PMCH，ALDH1A1，LPL和GHRH）途径有关的蛋白质的转录本。通过qPCR方法证实了RNA-seq结果。总之，下丘脑活动反映了3周龄鸡的密集生长，因为与生长激素轴和饱腹感中心调节相关的基因显示出增加的表达。

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