Detection of Sendai Virus and Pneumonia Virus of Mice by Use of Fluorogenic Nuclease Reverse Transcriptase Polymerase Chain Reaction Analysis

Wagner April M.; Loganbill Jessie K.; Besselsen David G.

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Detection of Sendai Virus and Pneumonia Virus of Mice by Use of Fluorogenic Nuclease Reverse Transcriptase Polymerase Chain Reaction Analysis

机译：荧光核酸酶逆转录酶聚合酶链反应分析检测小鼠仙台病毒和肺炎病毒

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Sendaivirusmayinduceacuterespiratorytractdiseaseinlaboratorymiceandisacommoncontaminantofbiologicalmaterials.Pneumoniavirusofmice(PVM)alsoinfectstherespiratorytractand,likeSendaivirus,mayinduceapersistentwastingdiseasesyndromeinimmunodeficientmice.Reversetranscriptase-polymerasechainreaction(RT-PCR)assayshaveprovenusefulfordetectionofSendaivirusandPVMinimmunodeficientanimalsandcontaminatedbiomaterials.FluorogenicnucleaseRT-PCRassays(fnRT-PCR)combineRT-PCRwithaninternalfluorogenichybridizationprobe,therebypotentiallyenhancingspecificityandeliminatingpost-PCRprocessing.Therefore,fnRT-PCRassaysspecificforSendaivirusandPVMweredevelopedbytargetingprimerandprobesequencestouniqueregionsoftheSendaivirusnucleocapsid(NP)geneandthePVMattachment(G)gene,respectively.TheSendaivirusandPVMfnRT-PCRassaysdetectedonlySendaivirusandPVM,respectively.NeitherassaydetectedothervirusesofthefamilyParamyxoviridaeorotherRNAvirusesthatnaturallyinfectrodents.ThefnRT-PCRassaysdetectedaslittleas10fgofSendaivirusRNAandonepicogramofPVMRNA,respectively,andtheSendaivirusfnRT-PCRassayhadcomparablesensitivitywhendirectlycomparedwiththemouseantibodyproductiontest.ThefnRT-PCRassayswerealsoabletodetectviralRNAinrespiratorytracttissuesandcageswipespecimenscollectedfromexperimentallyinoculatedC.B-17severecombinedimmunodeficientmice,butdidnotdetectviralRNAinage-andstrain-matchedmock-infectedmice.Inconclusion,thesefnRT-PCRassaysofferpotentiallyhigh-throughputdiagnosticassaystodetectSendaivirusandPVMinimmunodeficientmice,andtodetectSendaivirusincontaminatedbiologicalmaterials.

机译：Sendaivirusmayinduceacuterespiratorytractdiseaseinlaboratorymiceandisacommoncontaminantofbiologicalmaterials.Pneumoniavirusofmice（PVM）alsoinfectstherespiratorytractand，likeSendaivirus，mayinduceapersistentwastingdiseasesyndromeinimmunodeficientmice.Reversetranscriptase-polymerasechainreaction（RT-PCR）assayshaveprovenusefulfordetectionofSendaivirusandPVMinimmunodeficientanimalsandcontaminatedbiomaterials.FluorogenicnucleaseRT-PCRassays（fnRT-PCR）combineRT-PCRwithaninternalfluorogenichybridizationprobe，therebypotentiallyenhancingspecificityandeliminatingpost-PCRprocessing.Therefore，fnRT-PCRassaysspecificforSendaivirusandPVMweredevelopedbytargetingprimerandprobesequencestouniqueregionsoftheSendaivirusnucleocapsid（NP）geneandthePVMattachment（G）该基因分别仅检测到了仙台病毒和PVMfnRT-PCR检测方法。分别检测到了仙台病毒和PVM。这两种检测方法均未检测到自然感染啮齿动物的双亲病毒或其他RNA病毒。检测到的fnRT-PCR检测方法仅检测了10个fendf SendaiRNA donepicogramofPVMRNA分别andtheSendaivirusfnRT-PCRassayhadcomparablesensitivitywhendirectlycomparedwiththemouseantibodyproductiontest.ThefnRT-PCRassayswerealsoabletodetectviralRNAinrespiratorytracttissuesandcageswipespecimenscollectedfromexperimentallyinoculatedC.B-17severecombinedimmunodeficientmice，butdidnotdetectviralRNAinage-andstrain-matchedmock-infectedmice.Inconclusion，thesefnRT-PCRassaysofferpotentiallyhigh-throughputdiagnosticassaystodetectSendaivirusandPVMinimmunodeficientmice，andtodetectSendaivirusincontaminatedbiologicalmaterials。

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《Comparative Medicine》 |2003年第2期|共页
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Wagner April M.; Loganbill Jessie K.; Besselsen David G.;
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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1. Detection of Sendai virus and pneumonia virus of mice by use of fluorogenic nuclease reverse transcriptase polymerase chain reaction analysis [J] . Wagner AM, Loganbill JK, Besselsen DG Comparative Medicine . 2003,第2期

机译：荧光核酸酶逆转录酶聚合酶链反应分析检测小鼠仙台病毒和肺炎病毒
2. Detection of lymphocytic choriomeningitis virus by use of fluorogenic nuclease reverse transcriptase-polymerase chain reaction analysis [J] . Besselsen DG, Wagner AM, Loganbill JK Comparative Medicine . 2003,第1期

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3. Detection of Lymphocytic Choriomeningitis Virus by Use of Fluorogenic Nuclease Reverse Transcriptase-Polymerase Chain Reaction Analysis [J] . Besselsen David G., Wagner April M., Loganbill Jessie K. Comparative Medicine . 2003,第1期

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Detection of Sendai Virus and Pneumonia Virus of Mice by Use of Fluorogenic Nuclease Reverse Transcriptase Polymerase Chain Reaction Analysis

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