Deoxyribonucleic Acid Preparation in Polymerase Chain Reaction Genotyping of Transgenic Mice

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Deoxyribonucleic Acid Preparation in Polymerase Chain Reaction Genotyping of Transgenic Mice

机译：转基因小鼠聚合酶链反应基因分型中脱氧核糖核酸的制备

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BackgroundandPurpose:Inanattempttofindarapidandreproduciblemethodforroutinepolymerasechainreactiongenotypingoftransgenicmice,twonovelapproachesweredeveloped.brxmlns="http://pub2web.metastore.ingenta.coms/"/brMethods:Oneapproachallowedreproducibleamplificationfromcrudelysatesoftailsnips,usingathermallyactivatedpolymerase.Inasecondapproach,forsituationsinwhichnon-invasivetechniquesarenecessary,oralswabspecimenswereamplifiedafterDNAextractionbyuseofanisolationkit.Samplesfrom10transgenicfactorVIIIknockoutmiceweregenotypedafterprocessingbyuseoftheseandothermethods.brxmlns="http://pub2web.metastore.ingenta.coms/"/brResults:False-negativeresultswerenotobtainedbyuseofthetwonovelapproaches.Despitetheirrelativesimplicity,bothapproachesyieldedresultscomparabletothoseobtainedbyuseofproceduresknowntobereliable,suchasorganicextractionandaDNAextractionkit.brxmlns="http://pub2web.metastore.ingenta.coms/"/brConclusion:BothapproachesareusefulforPCR-amplificationofDNAfrommammaliansources.

机译：BackgroundandPurpose：Inanattempttofindarapidandreproduciblemethodforroutinepolymerasechainreactiongenotypingoftransgenicmice，twonovelapproachesweredeveloped 方法：。Oneapproachallowedreproducibleamplificationfromcrudelysatesoftailsnips，usingathermallyactivatedpolymerase.Inasecondapproach，forsituationsinwhichnon-invasivetechniquesarenecessary，oralswabspecimenswereamplifiedafterDNAextractionbyuseofanisolationkit.Samplesfrom10transgenicfactorVIIIknockoutmiceweregenotypedafterprocessingbyuseoftheseandothermethods 结论：两种方法都可用于PCR扩增哺乳动物来源的DNA s。

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《Comparative Medicine》 |2000年第3期|共页
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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1. Deoxyribonucleic acid preparation in polymerase Chain reaction genotyping of transgenic mice [J] . Klaus Zimmermann, Hans Peter Schwarz, Peter L.Turecek Comparative Medicine . 2000,第3期

机译：转基因小鼠聚合酶链反应基因分型中脱氧核糖核酸的制备。
2. A simple polymerase chain reaction assay for genotyping the retinaldegeneration mutation (Pdeb(rd1)) in FVB/N-derived transgenic mice [J] . Gimenez E, Montoliu L Laboratory Animals . 2001,第2期

机译：用于对FVB / N衍生的转基因小鼠视网膜变性突变（Pdeb（rd1））进行基因分型的简单聚合酶链反应法
3. Application of peptide nucleic acid to the direct detection of deoxyribonucleic acid amplified by polymerase chain reaction [J] . Sawata S, Ikebukuro K, Iida T, Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices . 1999,第4期

机译：肽核酸在直接检测聚合酶链反应扩增的脱氧核糖核酸中的应用
4. Polymerase chain reaction (PCR) and sequence specific oligonucleotide probes (SSOP) genotyping assay for detection of genes associated with rheumatoid arthritis and multiple sclerosis [C] . Nikolaou Konstantina, Kalatzis Fanis G., Giannakeas Nikolaos, 32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society . 2010

机译：聚合酶链反应（PCR）和序列特异性寡核苷酸探针（SSOP）基因分型测定法，用于检测与类风湿性关节炎和多发性硬化症相关的基因
5. Development and application of full-length and polymerase chain reaction-derived partial-length VP4- and VP7-specific nucleic acid probes for the differentiation of porcine rotavirus serotypes. [D] . Rosen, Blair Ira. 1993

机译：全长和聚合酶链反应衍生的部分长度VP4-和VP7特异性核酸探针在猪轮状病毒血清型分化中的开发和应用。
6. Serological Analysis of the Deoxyribonucleic Acid Polymerase of Avian Oncornaviruses I. Preparation and Characterization of Monospecific Antiserum with Purified Deoxyribonucleic Acid Polymerase [O] . K. F. Watson, R. C. Nowinski, A. Yaniv, 1972

机译：禽肿瘤病毒脱氧核糖核酸聚合酶的血清学分析I.纯化的脱氧核糖核酸聚合酶制备和鉴定单特异性抗血清
7. Molecular Diagnosis of Paramyxovirus Infection in Snakes Using Reverse Transcriptase–Polymerase Chain Reaction and Complementary Deoxyribonucleic Acid:Ribonucleic Acid in Situ Hybridization [O] . Matthew A. Sand, Kenneth S. Latimer, Christopher R. Gregory, 2004

机译：使用逆转录酶 - 聚合酶链反应和互换脱氧核酸的鼻子副Xovirus感染的分子诊断：核糖核原位杂交
8. Automation and integration of polymerase chain reaction with capillary electrophoresis for high throughput genotyping and disease diagnosis [R] . Zhang, N. 1999

机译：聚合酶链反应与毛细管电泳的自动化和整合，用于高通量基因分型和疾病诊断

Deoxyribonucleic Acid Preparation in Polymerase Chain Reaction Genotyping of Transgenic Mice

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