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首页> 外文期刊>Biology Open >Cell adhesion and sorting in embryoid bodies derived from N- or E-cadherin deficient murine embryonic stem cells
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Cell adhesion and sorting in embryoid bodies derived from N- or E-cadherin deficient murine embryonic stem cells

机译：N-或E-钙粘蛋白缺陷型鼠胚胎干细胞来源的胚状体中的细胞粘附和分选

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The primitive endoderm epithelial structure in mouse blastocysts forms following cell differentiation and subsequent sorting, and this two-step process can be reproduced in vitro using an embryoid body model. We found that in the chimeric embryoid bodies consisting of paired wildtype and E-cadherin null ES cells, the wildtype sorted to the center and were enveloped by the less adhesive E-cadherin null cells, in accord with Steinberg's hypothesis. However, wildtype and N-cadherin null ES cells intermixed and did not segregate, a situation that may be explained by Albert Harris' modified principle, which incorporates the unique properties of living cells. Furthermore, in chimeric embryoid bodies composed of N-cadherin and E-cadherin null ES cells, the two weakly interacting cell types segregated but did not envelop one another. Lastly, the most consistent and striking observation was that differentiated cells sorted to the surface and formed an enveloping layer, regardless of the relative cell adhesive affinity of any cell combination, supporting the hypothesis that the ability of the differentiated cells to establish apical polarity is the determining factor in surface sorting and positioning.
机译：小鼠胚泡中的原始内胚层上皮结构在细胞分化和随后的分选后形成，并且可以使用胚状体模型在体外复制此两步过程。我们发现，在由成对的野生型和E-cadherin无效ES细胞组成的嵌合胚状体中，野生型排序到中心，并被较少粘附的E-cadherin无效细胞所包裹，这与Steinberg的假设相符。但是，野生型和N-钙黏着蛋白无效的ES细胞混合在一起并且没有分离，这种情况可以用阿尔伯特·哈里斯（Albert Harris）修改后的原理加以解释，该原理结合了活细胞的独特特性。此外，在由N-钙粘着蛋白和E-钙粘着蛋白缺失的ES细胞组成的嵌合胚状体中，两种弱相互作用的细胞类型分离但彼此不包裹。最后，最一致和最显着的观察结果是，分化的细胞分选到表面并形成包裹层，而与任何细胞组合的相对细胞粘附亲和力无关，这支持了这样的假设：分化的细胞建立顶极的能力是确定表面分类和定位的因素。

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