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首页> 外文期刊>Biology Open >Bazooka/PAR3 is dispensable for polarity in Drosophila follicular epithelial cells
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Bazooka/PAR3 is dispensable for polarity in Drosophila follicular epithelial cells

机译：火箭筒/ PAR3可用于果蝇滤泡上皮细胞中的极性

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Apico-basal polarity is the defining characteristic of epithelial cells. In Drosophila , apical membrane identity is established and regulated through interactions between the highly conserved Par complex (Bazooka/Par3, atypical protein kinase C and Par6), and the Crumbs complex (Crumbs, Stardust and PATJ). It has been proposed that Bazooka operates at the top of a genetic hierarchy in the establishment and maintenance of apico-basal polarity. However, there is still ambiguity over the correct sequence of events and cross-talk with other pathways during this process. In this study, we reassess this issue by comparing the phenotypes of the commonly used baz4 and baz815-8 alleles with those of the so far uncharacterized bazXR11 and bazEH747 null alleles in different Drosophila epithelia. While all these baz alleles display identical phenotypes during embryonic epithelial development, we observe strong discrepancies in the severity and penetrance of polarity defects in the follicular epithelium: polarity is mostly normal in bazEH747 and bazXR11 while baz4 and baz815 -8 show loss of polarity, severe multilayering and loss of epithelial integrity throughout the clones. Further analysis reveals that the chromosomes carrying the baz4 and baz815-8 alleles may contain additional mutations that enhance the true baz loss-of-function phenotype in the follicular epithelium. This study clearly shows that Baz is dispensable for the regulation of polarity in the follicular epithelium, and that the requirement for key regulators of cell polarity is highly dependent on developmental context and cell type.
机译：顶基极极性是上皮细胞的定义特征。在果蝇中，通过高度保守的Par复合物（Bazooka / Par3，非典型蛋白激酶C和Par6）与Crumbs复合物（Crumbs，Stardust和PATJ）之间的相互作用来建立并调节顶膜身份。有人提出，火箭筒在建立和维持apico-基础极性的过程中处于遗传层次的顶部。但是，在此过程中，事件的正确顺序以及与其他途径的串扰仍然存在歧义。在这项研究中，我们通过比较常用的baz4和baz815-8等位基因的表型与迄今为止在不同果蝇上皮中尚未表征的bazXR11和bazEH747无效等位基因的表型来重新评估这个问题。尽管所有这些baz等位基因在胚胎上皮发育过程中均表现出相同的表型，但我们观察到了滤泡上皮中极性缺陷的严重性和穿透性存在巨大差异：bazEH747和bazXR11中的极性大多正常，而baz4和baz815 -8则显示极性丧失，严重整个克隆的多层化和上皮完整性的丧失。进一步的分析表明，携带baz4和baz815-8等位基因的染色体可能包含其他突变，这些突变会增强滤泡上皮细胞中真正的baz功能丧失表型。这项研究清楚地表明，Baz对于滤泡上皮中的极性调节是必不可少的，并且对细胞极性关键调节剂的要求高度依赖于发育背景和细胞类型。

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