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首页> 外文期刊>Biology Open >Homozygous and heterozygous GH transgenesis alters fatty acid composition and content in the liver of Amago salmon (Oncorhynchus masou ishikawae)
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Homozygous and heterozygous GH transgenesis alters fatty acid composition and content in the liver of Amago salmon (Oncorhynchus masou ishikawae)

机译：纯合子和杂合子GH转基因改变了Amago鲑鱼（Oncorhynchus masou ishikawae）肝脏中的脂肪酸组成和含量

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Growth hormone (GH) transgenic Amago ( Oncorhynchus masou ishikawae ), containing the sockeye GH1 gene fused with metallothionein-B promoter from the same species, were generated and the physiological condition through lipid metabolism compared among homozygous (Tg/Tg) and heterozygous GH transgenic (Tg/+) Amago and the wild type control (+/+). Previously, we have reported that the adipose tissue was generally smaller in GH transgenic fish compared to the control, and that the Δ-6 fatty acyl desaturase gene was down-regulated in the Tg/+ fish. However, fatty acid (FA) compositions have not been measured previously in these fish. In this study we compared the FAs composition and content in the liver using gas chromatography. Eleven kinds of FA were detected. The composition of saturated and monounsaturated fatty acids (SFA and MUFA) such as myristic acid (14:0), palmitoleic acid (16:1n-7), and cis-vaccenic acid (cis-18:1n-7) was significantly ( P 0.05) decreased in GH transgenic Amago. On the other hand, the composition of polyunsaturated fatty acids (PUFAs) such as linoleic acid (18:2n-6), arachidonic acid (20:4n-6), and docosapentaenoic acid (22:5n-3) was significantly ( P 0.05) increased. Levels of serum glucose and triacylglycerol were significantly ( P 0.05) decreased in the GH transgenics compared with +/+ fish. Furthermore, 3′-tag digital gene expression profiling was performed using liver tissues from Tg/Tg and +/+ fish, and showed that Mid1 interacting protein 1 (Mid1ip1), which is an important factor to activate Acetyl-CoA carboxylase (ACC), was down-regulated in Tg/Tg fish, while genes involved in FA catabolism were up-regulated, including long-chain-fatty-acid–CoA ligase 1 (ACSL1) and acyl-coenzyme A oxidase 3 (ACOX3). These data suggest that liver tissue from GH transgenic Amago showed starvation by alteration in glucose and lipid metabolism due to GH overexpression. The decrease of serum glucose suppressed Mid1ip1, and caused a decrease of de novo FA synthesis, resulting in a decrease of SFA and MUFA. This induced expression of ACSL1 and ACOX3 to produce energy through β-oxidation in the GH transgenic Amago.
机译：产生了转基因Amago（Oncorhynchus masou ishikawae），其包含与相同物种的金属硫蛋白-B启动子融合的红眼GH1基因，并通过脂质代谢比较了纯合（Tg / Tg）和杂合GH转基因的生理状况（Tg / +）Amago和野生型对照（+ / +）。先前，我们已经报道了与对照相比，GH转基因鱼的脂肪组织通常较小，并且Tg / +鱼中的Δ-6脂肪酰基去饱和酶基因被下调。但是，以前没有在这些鱼中测量过脂肪酸（FA）的成分。在这项研究中，我们使用气相色谱法比较了肝脏中FA的成分和含量。检测出11种FA。饱和和单不饱和脂肪酸（SFA和MUFA）的组成如肉豆蔻酸（14：0），棕榈油酸（16：1n-7）和顺式-庚酸（cis-18：1n-7）显着（ P <0.05）在GH转基因Amago中降低。另一方面，亚油酸（18：2n-6），花生四烯酸（20：4n-6）和二十二碳五烯酸（22：5n-3）等多不饱和脂肪酸（PUFA）的组成显着（P <0.05）增加。与+ / +鱼相比，GH转基因动物的血清葡萄糖和三酰甘油水平显着降低（P <0.05）。此外，使用来自Tg / Tg和+ / +鱼的肝脏组织进行了3'-tag数字基因表达谱分析，结果表明Mid1相互作用蛋白1（Mid1ip1）是激活乙酰辅酶A羧化酶（ACC）的重要因素在Tg / Tg鱼中被下调，而与FA分解代谢有关的基因被上调，包括长链脂肪酸CoA连接酶1（ACSL1）和酰基辅酶A氧化酶3（ACOX3）。这些数据表明，由于GH过表达，来自GH转基因Amago的肝组织由于葡萄糖和脂质代谢的改变而显示出饥饿。血清葡萄糖的减少抑制了Mid1ip1，并导致从头FA合成的减少，从而导致SFA和MUFA降低。这在GH转基因Amago中诱导了ACSL1和ACOX3的表达以通过β-氧化产生能量。

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