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首页> 外文期刊>Biology Open >Detection of changes in mitochondrial hydrogen sulfide in vivo in the fish model Poecilia mexicana (Poeciliidae)
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Detection of changes in mitochondrial hydrogen sulfide in vivo in the fish model Poecilia mexicana (Poeciliidae)

机译：鱼模型Poecilia mexicana（Poeciliidae）体内线粒体硫化氢变化的检测

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摘要

In this paper, we outline the use of a mitochondria-targeted ratiometric mass spectrometry probe, MitoA, to detectin vivochanges in mitochondrial hydrogen sulfide (H2S) inPoecilia mexicana(family Poeciliidae). MitoA is introduced via intraperitoneal injection into the animal and is taken up by mitochondria, where it reacts with H2S to form the product MitoN. The MitoN/MitoA ratio can be used to assess relative changes in the amounts of mitochondrial H2S produced over time. We describe the use of MitoA in the fish speciesP. mexicanato illustrate the steps for adopting the use of MitoA in a new organism, including extraction and purification of MitoA and MitoN from tissues followed by tandem mass spectrometry. In this proof-of-concept study we exposed H2S tolerantP. mexicanato 59?μM free H2S for 5?h, which resulted in increased MitoN/MitoA in brain and gills, but not in liver or muscle, demonstrating increased mitochondrial H2S levels in select tissues following whole-animal H2S exposure. This is the first time that accumulation of H2S has been observedin vivoduring whole-animal exposure to free H2S using MitoA.This article has an associated First Person interview with the first author of the paper.
机译：在本文中，我们概述了使用线粒体靶向比例质谱探针MitoA来检测墨西哥Poecilia mexicana（家族Poeciliidae）线粒体硫化氢（H2S）的体内变化。通过腹膜内注射将MitoA引入动物体内，并由线粒体吸收，然后与H2S反应形成MitoN产物。 MitoN / MitoA比率可用于评估随着时间推移产生的线粒体H2S量的相对变化。我们描述了在鱼类P中使用MitoA。 mexicanato说明了在新生物中采用MitoA的步骤，包括从组织中提取和纯化MitoA和MitoN，然后进行串联质谱分析。在此概念验证研究中，我们暴露了H2S耐受P。 mexicanato 59？μM的游离H2S持续5？h，导致脑和腮中MitoN / MitoA升高，但肝脏或肌肉中未见，表明全动物H2S暴露后某些组织中线粒体H2S水平升高。这是首次在全动物中使用MitoA暴露于游离H2S中，体内观察到了H2S的积累。本文与本文的第一作者进行了第一人称访谈。

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