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首页> 外文期刊>Biology Open >Unanchored ubiquitin chains do not lead to marked alterations in gene expression in Drosophila melanogaster
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Unanchored ubiquitin chains do not lead to marked alterations in gene expression in Drosophila melanogaster

机译：未锚定的遍在蛋白链不会导致果蝇黑色素基因表达的明显改变

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The small protein modifier ubiquitin regulates various aspects of cellular biology through its chemical conjugation onto proteins. Ubiquitination of proteins presents itself in numerous iterations, from a single mono-ubiquitination event to chains of poly-ubiquitin. Ubiquitin chains can be attached onto other proteins or can exist as unanchored species, i.e. free from another protein. Unanchored ubiquitin chains are thought to be deleterious to the cell and rapidly disassembled into mono-ubiquitin. We recently examined the toxicity and utilization of unanchored poly-ubiquitin inDrosophila melanogaster. We found that free poly-ubiquitin species are largely innocuous to flies and that free poly-ubiquitin can be controlled by being degraded by the proteasome or by being conjugated onto another protein as a single unit. Here, to explore whether an organismal defense is mounted against unanchored chains, we conducted RNA-Seq analyses to examine the transcriptomic impact of free poly-ubiquitin in the fly. We found ～90 transcripts whose expression is altered in the presence of different types of unanchored poly-ubiquitin. The set of genes identified was essentially devoid of ubiquitin-, proteasome-, or autophagy-related components. The seeming absence of a large and multipronged response to unanchored poly-ubiquitin supports the conclusion that these species need not be toxicin vivoand underscores the need to re-examine the role of free ubiquitin chains in the cell.
机译：小型蛋白质修饰剂泛素通过其与蛋白质的化学结合来调节细胞生物学的各个方面。从单个单泛素化事件到聚泛素链，蛋白质的泛素化以无数次迭代呈现。泛素链可以连接到其他蛋白质上，也可以作为非锚定物种存在，即不含其他蛋白质。未锚定的泛素链被认为对细胞有害，并迅速分解成单泛素。我们最近检查了无固定的聚泛素在果蝇中的毒性和利用。我们发现，游离的多聚泛素种类对果蝇是无害的，并且可以通过被蛋白酶体降解或通过与单个单元结合到另一种蛋白质上来控制游离的多聚泛素。在这里，为了探讨是否对未锚定的链进行了生物防御，我们进行了RNA-Seq分析，以检查游离聚泛素在飞行中的转录组学影响。我们发现了〜90个转录物，其表达在不同类型的未锚定聚泛素存在下发生了变化。鉴定出的基因集基本上没有泛素，蛋白酶体或自噬相关成分。似乎没有对未锚定的聚遍在蛋白产生大而多管齐下的反应，支持了以下结论：这些物种无需在体内具有毒性，并强调了需要重新检查细胞中游离泛素链的作用。

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