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首页> 外文期刊>Biology Open >Pharmacologic inhibition of phospholipase C in the brain attenuates early memory formation in the honeybee (Apis mellifera L.)
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Pharmacologic inhibition of phospholipase C in the brain attenuates early memory formation in the honeybee (Apis mellifera L.)

机译：大脑中磷脂酶C的药理抑制作用会削弱蜜蜂（Apis mellifera L.）的早期记忆形成

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Although the molecular mechanisms involved in learning and memory in insects have been studied intensively, the intracellular signaling mechanisms involved in early memory formation are not fully understood. We previously demonstrated thatphospholipase C epsilon(PLCe), whose product is involved in calcium signaling, is almost selectively expressed in the mushroom bodies, a brain structure important for learning and memory in the honeybee. Here, we pharmacologically examined the role of phospholipase C (PLC) in learning and memory in the honeybee. First, we identified four genes for PLC subtypes in the honeybee genome database. Quantitative reverse transcription-polymerase chain reaction revealed that, among these four genes, three, includingPLCe, were expressed higher in the brain than in sensory organs in worker honeybees, suggesting their main roles in the brain. Edelfosine and neomycin, pan-PLC inhibitors, significantly decreased PLC activities in homogenates of the brain tissues. These drugs injected into the head of foragers significantly attenuated memory acquisition in comparison with the control groups, whereas memory retention was not affected. These findings suggest that PLC in the brain is involved in early memory formation in the honeybee. To our knowledge, this is the first report of a role for PLC in learning and memory in an insect.
机译：尽管已深入研究了昆虫学习和记忆的分子机制，但尚未完全了解早期记忆形成的细胞内信号传导机制。我们以前证明磷脂酶C epsilon（PLCe）的产物参与钙信号传导，几乎选择性地在蘑菇体内表达，蘑菇的大脑结构对于蜜蜂的学习和记忆很重要。在这里，我们用药理学的方法研究了磷脂酶C（PLC）在蜜蜂学习和记忆中的作用。首先，我们在蜜蜂基因组数据库中确定了PLC亚型的四个基因。定量逆转录-聚合酶链反应显示，在这四个基因中，三个基因（包括PLCe）在大脑中的表达高于在工蜂中的感觉器官，表明它们在大脑中的主要作用。泛PLC抑制剂Edelfosine和neomycin显着降低了脑组织匀浆中的PLC活性。与对照组相比，注入觅食者头部的这些药物显着降低了记忆获得能力，而记忆保持能力却没有受到影响。这些发现表明，大脑中的PLC参与了蜜蜂的早期记忆形成。据我们所知，这是PLC在昆虫学习和记忆中的作用的首次报道。

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