Detection of Rodent Helicobacter spp. by Use of Fluorogenic Nuclease Polymerase Chain Reaction Assays

Drazenovich Nicole L.; Franklin Craig L.; Livingston Robert S.; Besselsen David G.

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Detection of Rodent Helicobacter spp. by Use of Fluorogenic Nuclease Polymerase Chain Reaction Assays

机译：啮齿动物幽门螺杆菌的检测。通过荧光核酸酶聚合酶链反应分析

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Polymerasechainreaction(PCR)analysisisthestandardmethodfordetectionofHelicobacterspp.infectionsinlaboratoryrodents,withH.hepaticus,H.bilis,andH.typhloniusconsideredprimarypathogens.FluorogenicnucleasePCRassaysthatdetectallknownrodentHelicobacterspp.,orthatspecificallydetectH.hepaticus,H.bilis,orH.typhloniusweredevelopedtoeliminatepost-PCRprocessing,enhancespecificity,andprovidequantitativedataonstartingtemplateconcentration.EachfluorogenicPCRassaydetectedaminimumof10copiesoftargettemplate,hadcomparableorgreatersensitivitywhencompareddirectlywithcorollarygeldetectionPCRassays,anddetectedonlytargetedspecieswhennumerousHelicobacterspp.andotherentericbacteriawereanalyzed.FluorogenicnucleasePCRanalysisoffecalDNAsamplesobtainedfromnumerouslaboratorymicesourcesdetectedallsampleswithpositiveresultsbyuseofHelicobacterspp.,H.hepaticus,H.bilis,and/orH.typhloniusgeldetectionPCRanalysis,exceptforonesamplethathadpositiveresultsbyH.typhloniusgeldetectionPCRbutnegativeresultsbyH.typhloniusfluorogenicnucleasePCRanalysis.AmongfecalDNAsamplesthatwereHelicobacterspp.negativebyuseofallgeldetectionPCRassays,thefluorogenicnucleasePCRassaysdetectedtargettemplateinonlyonesamplethatwaspositivebyuseoftheHelicobacterspp.andtheH.bilisfluorogenicnucleasePCRassays.Inconclusion,fluorogenicnucleasePCRassaysprovidesensitive,specific,andhigh-throughputdiagnosticassaysfordetectionofHelicobacterspp.,H.hepaticus,H.bilis,andH.typhloniusinlaboratoryrodents,andthequantitativedatageneratedbytheseassaysmakethempotentiallyusefulforbacterialloaddetermination.

机译：Polymerasechainreaction（PCR）analysisisthestandardmethodfordetectionofHelicobacterspp.infectionsinlaboratoryrodents，withH.hepaticus，H.bilis，andH.typhloniusconsideredprimarypathogens.FluorogenicnucleasePCRassaysthatdetectallknownrodentHelicobacterspp。，orthatspecificallydetectH.hepaticus，H.bilis，orH.typhloniusweredevelopedtoeliminatepost-PCRprocessing，enhancespecificity，andprovidequantitativedataonstartingtemplateconcentration.EachfluorogenicPCRassaydetectedaminimumof10copiesoftargettemplate，hadcomparableorgreatersensitivitywhencompareddirectlywithcorollarygeldetectionPCRassays，anddetectedonlytargetedspecieswhennumerousHelicobacterspp.andotherentericbacteriawereanalyzed.FluorogenicnucleasePCRanalysisoffecalDNAsamplesobtainedfromnumerouslaboratorymicesourcesdetectedallsampleswithpositiveresultsbyuseofHelicobacterspp ，H. bilibilis和/或H.typhlonius凝胶检测PCR分析，除了一个样品因H. typhlonius凝胶检测PCR产生阳性结果而H. typhloniusfluorgenicn阴性ucleasePCRanalysis.AmongfecalDNAsamplesthatwereHelicobacterspp.negativebyuseofallgeldetectionPCRassays，thefluorogenicnucleasePCRassaysdetectedtargettemplateinonlyonesamplethatwaspositivebyuseoftheHelicobacterspp.andtheH.bilisfluorogenicnucleasePCRassays.Inconclusion，fluorogenicnucleasePCRassaysprovidesensitive，具体，andhigh-throughputdiagnosticassaysfordetectionofHelicobacterspp，H.hepaticus，H.bilis，andH.typhloniusinlaboratoryrodents，andthequantitativedatageneratedbytheseassaysmakethempotentiallyusefulforbacterialloaddetermination。

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《Comparative Medicine》 |2002年第4期|共页
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Drazenovich Nicole L.; Franklin Craig L.; Livingston Robert S.; Besselsen David G.;
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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Detection of Rodent Helicobacter spp. by Use of Fluorogenic Nuclease Polymerase Chain Reaction Assays

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