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首页> 外文期刊>Biology Open >The labial gene is required to terminate proliferation of identified neuroblasts in postembryonic development of the Drosophila brain
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The labial gene is required to terminate proliferation of identified neuroblasts in postembryonic development of the Drosophila brain

机译：在果蝇大脑胚胎后发育过程中，需要唇酸基因来终止已鉴定的神经母细胞的增殖

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The developing brain of Drosophila has become a useful model for studying the molecular genetic mechanisms that give rise to the complex neuronal arrays that characterize higher brains in other animals including mammals. Brain development in Drosophila begins during embryogenesis and continues during a subsequent postembryonic phase. During embryogenesis, the Hox gene labial is expressed in the developing tritocerebrum, and labial loss-of-function has been shown to be associated with a loss of regional neuronal identity and severe patterning defects in this part of the brain. However, nothing is known about the expression and function of labial , or any other Hox gene, during the postembryonic phase of brain development, when the majority of the neurons in the adult brain are generated. Here we report the first analysis of Hox gene action during postembryonic brain development in Drosophila . We show that labial is expressed initially in six larval brain neuroblasts, of which only four give rise to the labial expressing neuroblast lineages present in the late larval brain. Although MARCM-based clonal mutation of labial in these four neuroblast lineages does not result in an obvious phenotype, a striking and unexpected effect of clonal labial loss-of-function does occur during postembryonic brain development, namely the formation of two ectopic neuroblast lineages that are not present in wildtype brains. The same two ectopic neuroblast lineages are also observed following cell death blockage and, significantly, in this case the resulting ectopic lineages are Labial-positive. These findings imply that labial is required in two specific neuroblast lineages of the wildtype brain for the appropriate termination of proliferation through programmed cell death. Our analysis of labial function reveals a novel cell autonomous role of this Hox gene in shaping the lineage architecture of the brain during postembryonic development.
机译：果蝇的大脑发育已成为研究分子遗传机制的有用模型，这些分子遗传机制产生了复杂的神经元阵列，这些阵列表征了包括哺乳动物在内的其他动物的高级大脑。果蝇的大脑发育在胚胎发生期间开始，并在随后的胚胎后阶段持续进行。在胚胎发生过程中，Hox基因唇在发育中的三角脑中表达，并且唇的功能丧失已被证明与该区域大脑局部神经元身份的丧失和严重的模式缺陷有关。但是，在大脑发育的胚后阶段，当成年大脑中的大多数神经元都产生时，关于唇肌或任何其他Hox基因的表达和功能的信息一无所知。在这里，我们报告果蝇胚后大脑发育过程中Hox基因作用的首次分析。我们显示，最初在六个幼虫脑神经母细胞中表达了唇神经，其中只有四个引起了后期幼虫脑中存在的唇形表达神经母细胞谱系。尽管在这四个成神经细胞谱系中基于MARCM的唇部克隆突变不会导致明显的表型，但在胚胎后脑发育过程中确实发生了克隆性唇功能丧失的惊人和意想不到的效果，即形成了两个异位成神经细胞谱系在野生型大脑中不存在。细胞死亡受阻后也观察到相同的两个异位神经母细胞谱系，并且在这种情况下，产生的异位谱系明显呈阴唇阳性。这些发现暗示在野生型大脑的两个特定成神经细胞谱系中需要唇唇以通过程序性细胞死亡适当终止增殖。我们对唇功能的分析揭示了该Hox基因在胚胎后发育过程中塑造大脑谱系结构中的新型细胞自治作用。

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