...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biology Open >Blocking autophagy enhances the pro-apoptotic effect of bufalin on human gastric cancer cells through endoplasmic reticulum stress
【24h】

Blocking autophagy enhances the pro-apoptotic effect of bufalin on human gastric cancer cells through endoplasmic reticulum stress

机译：阻断自噬通过内质网应激增强蟾蜍灵对人胃癌细胞的促凋亡作用

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bufalin has been used to treat cancer for several years. However, the molecular mechanisms for its anti-tumor function are not fully understood. This work aimed to investigate the effect of bufalin on the proliferation and apoptosis of human gastric cancer (HGC) cells and the roles of endoplasmic reticulum (ER) stress and autophagy in bufalin-induced apoptosis. HGC cell lines, SGC7901 and BGC823, were treated with different concentrations of bufalin or 80?nmol/l bufalin for 1, 2, 3 and 4?days. Cell counting kit-8 (CCK-8) assay and direct cell counting method were used to detect proliferation. Cell cycle arrest and apoptosis was detected using flow cytometry. Protein levels of caspase-3, -8, Bax/Bcl-2, Beclin-1, LC3, inositol-requiring enzyme 1 (IRE1) and C/EBP homologous protein (CHOP) were determined using western blotting. Autophagy was blocked using 3-methyladenine (3MA) or Atg5 siRNA to evaluate the effect of autophagy on bufalin-induced apoptosis. The IRE1 and CHOP were knocked down using specific siRNA to determine the pathway involved in bufalin-induced autophagy. It was found that bufalin significantly suppressed proliferation of SGC7901 and BGC823 cells and induced apoptosis in a time- and dose-dependent manner. The mechanism responsible for bufalin-induced apoptosis was the formation of ER stress via the IRE1-JNK pathway. Moreover, autophagy was activated during ER stress, and blocking autophagy significantly exacerbated bufalin-induced apoptosis.
机译：蟾蜍灵已被用于治疗癌症数年。但是，其抗肿瘤功能的分子机制尚未完全了解。这项工作旨在调查蟾蜍灵对人胃癌（HGC）细胞增殖和凋亡的影响，以及内质网（ER）应激和自噬在蟾蜍灵诱导的凋亡中的作用。将HGC细胞系SGC7901和BGC823用不同浓度的蟾蜍灵或80？nmol / l蟾蜍灵处理1、2、3和4天。使用细胞计数试剂盒8（CCK-8）测定法和直接细胞计数法检测增殖。使用流式细胞仪检测细胞周期停滞和凋亡。使用蛋白质印迹法测定caspase-3，-8，Bax / Bcl-2，Beclin-1，LC3，肌醇需要酶1（IRE1）和C / EBP同源蛋白（CHOP）的蛋白水平。使用3-甲基腺嘌呤（3MA）或Atg5 siRNA阻断自噬，以评估自噬对蟾蜍灵诱导的细胞凋亡的影响。使用特异性siRNA敲低IRE1和CHOP，以测定蟾蜍精诱导的自噬过程。发现蟾蜍灵显着抑制SGC7901和BGC823细胞的增殖并以时间和剂量依赖性方式诱导细胞凋亡。 bufalin诱导细胞凋亡的机制是通过IRE1-JNK途径形成ER应激。此外，内质网应激期间自噬被激活，阻断自噬显着加剧了蟾蜍灵诱导的细胞凋亡。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号