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首页> 外文期刊>Comparative and functional genomics >The β-Lactamase Gene Profile and a Plasmid-Carrying Multiple Heavy Metal Resistance Genes of Enterobacter cloacae
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The β-Lactamase Gene Profile and a Plasmid-Carrying Multiple Heavy Metal Resistance Genes of Enterobacter cloacae

机译：泄殖腔肠杆菌的β-内酰胺酶基因谱和携带多个重金属抗性的质粒

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In this work, by high-throughput sequencing, antibiotic resistance genes, including class A (blaCTX-M, blaZ, blaTEM, blaVEB, blaKLUC, and blaSFO), class C (blaSHV, blaDHA, blaMIR, blaAZECL-29, and blaACT), and class D (blaOXA) β-lactamase genes, were identified among the pooled genomic DNA from 212 clinical Enterobacter cloacae isolates. Six blaMIR-positive E. cloacae strains were identified, and pulsed-field gel electrophoresis (PFGE) showed that these strains were not clonally related. The complete genome of the blaMIR-positive strain (Y546) consisted of both a chromosome (4.78 Mb) and a large plasmid pY546 (208.74 kb). The extended-spectrum β-lactamases (ESBLs) (blaSHV-12 and blaCTX-M-9a) and AmpC (blaMIR) were encoded on the chromosome, and the pY546 plasmid contained several clusters of genes conferring resistance to metals, such as copper (pco), arsenic (ars), tellurite (ter), and tetrathionate (ttr), and genes encoding many divalent cation transporter proteins. The comparative genomic analyses of the whole plasmid sequence and of the heavy metal resistance gene-encoding regions revealed that the plasmid sequences of Klebsiella pneumoniae (such as pKPN-332, pKPN-3967, and pKPN-262) shared the highest similarity with those of pY546. It may be concluded that a variety of β-lactamase genes present in E. cloacae which confer resistance to β-lactam antibiotics and the emergence of plasmids carrying heavy metal resistance genes in clinical isolates are alarming and need further surveillance.
机译：在这项工作中，通过高通量测序，获得了抗生素抗性基因，包括A类（blaCTX-M，blaZ，blaTEM，blaVEB，blaKLUC和blaSFO），C类（blaSHV，blaDHA，blaMIR，blaAZECL-29和blaACT） ，和D类（blaOXA）β-内酰胺酶基因，从212株阴沟肠杆菌临床分离株的集合基因组DNA中鉴定出来。鉴定出六株blaMIR阳性阴沟肠杆菌菌株，脉冲场凝胶电泳（PFGE）显示这些菌株无克隆相关性。 blaMIR阳性菌株（Y546）的完整基因组由一条染色体（4.78 Mb）和一个大质粒pY546（208.74 kb）组成。染色体上编码了广谱β-内酰胺酶（ESBLs）（blaSHV-12和blaCTX-M-9a）和AmpC（blaMIR），pY546质粒包含多个基因簇，这些基因簇对金属（例如铜）具有抗性（ pco），砷（ars），亚碲酸盐（ter）和四硫代酸盐（ttr），以及编码许多二价阳离子转运蛋白的基因。对整个质粒序列和重金属抗性基因编码区进行的比较基因组分析表明，肺炎克雷伯氏菌的质粒序列（如pKPN-332，pKPN-3967和pKPN-262）与HBV的同源性最高。 pY546。可以得出结论，阴沟肠杆菌中存在多种赋予β-内酰胺抗生素耐药性的β-内酰胺酶基因，并且临床分离物中带有重金属抗性基因的质粒的出现令人震惊，需要进一步监测。

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