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首页> 外文期刊>Comparative and functional genomics >SCAR Marker for Gender Identification in Date Palm (Phoenix dactylifera L.) at the Seedling Stage
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SCAR Marker for Gender Identification in Date Palm (Phoenix dactylifera L.) at the Seedling Stage

机译：SCAR标记，用于在枣树期鉴定枣椰树（Phoenix dactylifera L.）中的性别

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Date palm (Phoenix dactylifera L.) is cultivated in arid and semiarid regions worldwide. Given the dioecious nature of this plant, gender identification is very important at the seedling stage. Molecular markers are very effective tools that help in gender identification at this stage. A sequence characterized amplified region (SCAR) marker linked to sex-specific regions in the genome of date palm was developed. Of the 300 tested randomly amplified polymorphic DNA (RAPD) primers, only one primer (OPC-06) produced reproducible band (294 bp) in male plants. The PCR product of this primer was cloned and sequenced. The specific primers were synthesized for amplification of a 186 bp fragment in male date palm plants. These primers were validated in male and female date palm plants, wherein the designed SCAR marker was reported only in male plants and no amplification was observed in female plants. The developed SCAR marker was used with seedlings of date palm and proved very effective in identification of gender.
机译：枣棕榈（Phoenix dactylifera L.）在世界各地的干旱和半干旱地区种植。鉴于这种植物的雌雄异株特性，在苗期鉴定性别非常重要。分子标记是非常有效的工具，可在此阶段帮助性别识别。开发了与枣椰基因组中性别特异性区域相关的序列特征扩增区域（SCAR）标记。在300种经测试的随机扩增多态性DNA（RAPD）引物中，只有一种引物（OPC-06）在雄性植物中产生了可重现的条带（294 bp）。对该引物的PCR产物进行克隆和测序。合成了特异性引物，用于在雄性枣椰子植物中扩增186 bp的片段。这些引物在雄性和雌性枣椰子植物中得到验证，其中仅在雄性植物中报道了设计的SCAR标记，而在雌性植物中未观察到扩增。发达的SCAR标记用于枣椰树的幼苗，证明对性别鉴定非常有效。

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