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首页> 外文期刊>Comparative and functional genomics >Topological Characterization of Human and Mouse m5C Epitranscriptome Revealed by Bisulfite Sequencing
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Topological Characterization of Human and Mouse m5C Epitranscriptome Revealed by Bisulfite Sequencing

机译：亚硫酸氢盐测序揭示人和小鼠m5C转录组的拓扑特征

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Background. Compared with the well-studied 5-methylcytosine (m5C) in DNA, the role and topology of epitranscriptome m5C remain insufficiently characterized. Results. Through analyzing transcriptome-wide m5C distribution in human and mouse, we show that the m5C modification is significantly enriched at 5 untranslated regions (5UTRs) of mRNA in human and mouse. With a comparative analysis of the mRNA and DNA methylome, we demonstrate that, like DNA methylation, transcriptome m5C methylation exhibits a strong clustering effect. Surprisingly, an inverse correlation between mRNA and DNA m5C methylation is observed at CpG sites. Further analysis reveals that RNA m5C methylation level is positively correlated with both RNA expression and RNA half-life. We also observed that the methylation level of mitochondrial RNAs is significantly higher than RNAs transcribed from the nuclear genome. Conclusions. This study provides an in-depth topological characterization of transcriptome-wide m5C modification by associating RNA m5C methylation patterns with transcriptional expression, DNA methylations, RNA stabilities, and mitochondrial genome.
机译：背景。与DNA中经过深入研究的5-甲基胞嘧啶（m5C）相比，表位转录组m5C的作用和拓扑结构仍然不够充分。结果。通过分析在人类和小鼠中转录组范围内的m5C分布，我们显示m5C修饰在人类和小鼠的mRNA的5个非翻译区（5UTRs）处显着富集。通过对mRNA和DNA甲基化组的比较分析，我们证明，与DNA甲基化一样，转录组m5C甲基化表现出很强的聚类作用。出乎意料的是，在CpG位点观察到mRNA与DNA m5C甲基化之间呈负相关。进一步的分析表明，RNA m5C甲基化水平与RNA表达和RNA半衰期均呈正相关。我们还观察到线粒体RNA的甲基化水平明显高于从核基因组转录的RNA。结论。这项研究通过将RNA m5C甲基化模式与转录表达，DNA甲基化，RNA稳定性和线粒体基因组相关联，提供了转录组范围m5C修饰的深入拓扑学表征。

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