...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biology Open >Phosphatase-regulated recruitment of the spindle- and kinetochore-associated (Ska) complex to kinetochores
【24h】

Phosphatase-regulated recruitment of the spindle- and kinetochore-associated (Ska) complex to kinetochores

机译：磷酸酶调节的纺锤体和线粒体（Ska）复合体向动植物的募集

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Kinetochores move chromosomes on dynamic spindle microtubules and regulate signaling of the spindle checkpoint. The spindle- and kinetochore-associated (Ska) complex, a hexamer composed of two copies of Ska1, Ska2 and Ska3, has been implicated in both roles. Phosphorylation of kinetochore components by the well-studied mitotic kinases Cdk1, Aurora B, Plk1, Mps1, and Bub1 regulate chromosome movement and checkpoint signaling. Roles for the opposing phosphatases are more poorly defined. Recently, we showed that the C terminus of Ska1 recruits protein phosphatase 1 (PP1) to kinetochores. Here we show that PP1 and protein phosphatase 2A (PP2A) both promote accumulation of Ska at kinetochores. Depletion of PP1 or PP2A by siRNA reduces Ska binding at kinetochores, impairs alignment of chromosomes to the spindle midplane, and causes metaphase delay or arrest, phenotypes that are also seen after depletion of Ska. Artificial tethering of PP1 to the outer kinetochore protein Nuf2 promotes Ska recruitment to kinetochores, and it reduces but does not fully rescue chromosome alignment and metaphase arrest defects seen after Ska depletion. We propose that Ska has multiple functions in promoting mitotic progression and that kinetochore-associated phosphatases function in a positive feedback cycle to reinforce Ska complex accumulation at kinetochores.
机译：动植物在动态纺锤体微管上移动染色体并调节纺锤体检查点的信号传导。纺锤体和线粒体相关的（Ska）复合物是由两个拷贝的Ska1，Ska2和Ska3组成的六聚体，两者都有牵连作用。深入研究有丝分裂激酶Cdk1，Aurora B，Plk1，Mps1和Bub1形成的线粒体成分的磷酸化调节染色体运动和检查点信号传导。相对磷酸酶的作用定义较差。最近，我们显示Ska1的C末端募集蛋白磷酸酶1（PP1）到动植物。在这里，我们显示PP1和蛋白磷酸酶2A（PP2A）都促进动植物的Ska积累。 siRNA耗尽PP1或PP2A会减少动植物的Ska结合，削弱染色体与纺锤体中平面的对准，并导致中期延迟或停滞，这是在Ska耗尽后也能看到的表型。 PP1与外部动粒蛋白Nuf2的人工束缚可将Ska募集到动植物，并减少但不能完全挽救Ska耗尽后出现的染色体排列和中期停滞缺陷。我们建议，Ska具有促进有丝分裂进程的多种功能，并且动线粒体相关的磷酸酶在正反馈循环中可增强Ska复合体在动植物上的积累。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号