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首页> 外文期刊>Biology Open >Architectural delineation and molecular identification of extracellular matrix in ascidian embryos and larvae
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Architectural delineation and molecular identification of extracellular matrix in ascidian embryos and larvae

机译：海鞘胚胎和幼虫细胞外基质的结构描述和分子鉴定

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The extracellular matrix (ECM) not only provides essential physical scaffolding for cellular constituents but also initiates crucial biochemical and biomechanical cues that are required for tissue morphogenesis. In this study, we utilized wheat germ agglutinin (WGA) staining to characterize the ECM architecture in ascidian embryos and larvae. The results showed three distinct populations of ECM presenting in?Ciona?embryogenesis: the outer layer localized at the surface of embryo, an inner layer of notochord sheath and the apical ECM secreted by the notochord. To further elucidate the precise structure of?Ciona?embryonic ECM, we employed scanning and transmission electron microscopy, and found that the outer membrane was relatively thick with short fibres, whereas the ECM layer in notochord sheath was not as thick as the outer membrane but more regular arranged; the lumen between notochord cells was hydrostatic and sticky. Then, we used the RNA sequencing data from the embryos and larvae of?Ciona savignyi?to identify ECM genes and acquire their expression patterns. We identified 115 unigenes as 67 ECM genes, and 77 unigenes showed dynamic expression changes between different stages. Our results reveal the architecture, molecular composition and dynamic expression profile of ECM in ascidian embryogenesis, and may increase understanding of the function of the ECM in chordate development.
机译：细胞外基质（ECM）不仅为细胞成分提供了必要的物理支架，而且还引发了组织形态发生所需的重要生化和生物力学线索。在这项研究中，我们利用小麦胚芽凝集素（WGA）染色来表征海鞘胚胎和幼虫中的ECM结构。结果表明，三种不同的ECM群体参与了Ciona胚的发生：外层位于胚胎表面，内线鞘的内层和由内脊线分泌的顶端ECM。为了进一步阐明“ Ciona”胚胚ECM的精确结构，我们采用扫描和透射电镜观察，发现外膜相对较厚，纤维短，而脊索鞘中的ECM层不如外膜厚，而是安排得井井有条脊索细胞之间的内腔具有静水力和粘性。然后，我们使用了来自中华绒螯蟹（Ciona savignyi）的幼虫和幼虫的RNA测序数据来鉴定ECM基因并获得其表达模式。我们鉴定出115个单基因为67个ECM基因，其中77个单基因在不同阶段之间表现出动态表达变化。我们的研究结果揭示了ECM在海藻胚胎发生中的结构，分子组成和动态表达谱，并可能增加对ECM在氯酸盐发育中功能的了解。

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