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The Complete Mitochondrial Genome ofCtenoptilum vasava(Lepidoptera: Hesperiidae: Pyrginae) and Its Phylogenetic Implication

机译:剑叶番荔枝线粒体的完整线粒体基因组及其系统发生学意义

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We here report the first complete mitochondrial (mt) genome of a skipper,Ctenoptilum vasavaMoore, 1865 (Lepidoptera: Hesperiidae: Pyrginae). The mt genome of the skipper is a circular molecule of 15,468 bp, containing 2 ribosomal RNA genes, 24 putative transfer RNA (tRNA), genes including an extra copy of trnS (AGN) and a tRNA-like insertion trnL (UUR), 13 protein-coding genes and an AT-rich region. All protein-coding genes (PCGs) are initiated by ATN codons and terminated by the typical stop codon TAA or TAG, except for COII which ends with a single T. The intergenic spacer sequence between trnS (AGN) and ND1 genes also contains the ATACTAA motif. The AT-rich region of 429 bp is comprised of nonrepetitive sequences, including the motif ATAGA followed by an 19 bp poly-T stretch, a microsatellite-like (AT)3(TA)9element next to the ATTTA motif, an 11 bp poly-A adjacent to tRNAs. Phylogenetic analyses (ML and BI methods) showed that Papilionoidea is not a natural group, and Hesperioidea is placed within the Papilionoidea as a sister to ((Pieridae+Lycaenidae)+Nymphalidae) while Papilionoidae is paraphyletic to Hesperioidea. This result is remarkably different from the traditional view where Papilionoidea and Hesperioidea are considered as two distinct superfamilies.
机译:我们在这里报告了一个船长的第一个完整的线粒体(mt)基因组,即Ctenoptilum vasavaMoore,1865年(鳞翅目:Hesperiidae:Pyrginae)。船长的mt基因组是一个15468 bp的环状分子,包含2个核糖体RNA基因,24个推定的转移RNA(tRNA),包括额外拷贝的trnS(AGN)和类似tRNA的插入trnL(UUR)的基因,13蛋白质编码基因和一个富含AT的区域。所有蛋白质编码基因(PCG)均由ATN密码子启动,并由典型的终止密码子TAA或TAG终止,除了以单个T结束的COII外。trnS(AGN)和ND1基因之间的基因间隔区序列也包含ATACTAA主题。富含429 bp的AT的区域由非重复序列组成,包括ATAGA基序,其后是19 bp的poly-T延伸序列,紧接ATTTA基序的微卫星状(AT)3(TA)9元素,一个11 bp的多核苷酸-与tRNA相邻的-A。系统发育分析(ML和BI方法)表明,蝶形花科不是天然的,并且将蝶形花科作为((Pieridae + Lycaenidae)+ Nymphalidae)的姊妹置于蝶形花科内,而蝶形花科则是蝶形花科的副生。这一结果与传统观点显着不同,在传统观点中,蝶形花纲和橙皮菊纲被认为是两个不同的超家族。

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