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首页> 外文期刊>Comparative and functional genomics >The Application of Restriction Landmark Genome Scanning Method for Surveillance of Non-Mendelian Inheritance inF1Hybrids
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The Application of Restriction Landmark Genome Scanning Method for Surveillance of Non-Mendelian Inheritance inF1Hybrids

机译：限制性地标基因组扫描方法在F1杂种非孟德尔遗传监测中的应用

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We analyzed inheritance of DNA methylation in reciprocalF1hybrids (subsp.japonicacv. Nipponbare×subsp.indicacv. Kasalath) of rice (Oryza sativaL.) using restriction landmark genome scanning (RLGS), and detected differing RLGS spots between the parents and reciprocalF1hybrids.MspI/HpaII restriction sites in the DNA from these different spots were suspected to be heterozygously methylated in the Nipponbare parent. These spots segregated inF1plants, but did not segregate in selfed progeny of Nipponbare, showing non-Mendelian inheritance of the methylation status. As a result of RT-PCR and sequencing, a specific allele of the gene nearest to the methylated sites was expressed in reciprocalF1plants, showing evidence of biased allelic expression. These results show the applicability of RLGS for scanning of non-Mendelian inheritance of DNA methylation and biased allelic expression.
机译：我们使用限制性地标基因组扫描（RLGS）分析了水稻（Oryza sativaL。）的倒数F1杂种（japonicacv。Nipponbare×变种亚种。Kasalath）中DNA甲基化的遗传，并检测了亲本和倒数F1杂种。怀疑来自Nipponbare亲本的来自这些不同斑点的DNA中的HpaII限制性酶切位点杂合了甲基化。这些斑点在F1植物中分离，但在日本晴的自交后代中没有分离，表明甲基化状态的非孟德尔遗传。 RT-PCR和测序的结果是，最接近甲基化位点的基因的特定等位基因在倒数F1植物中表达，显示了等位基因表达偏向的证据。这些结果表明，RLGS适用于扫描DNA甲基化的非孟德尔遗传和偏倚的等位基因表达。

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