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首页> 外文期刊>Comparative and functional genomics >Analysis of Transcripts Expressed in One-Day-Old Larvae and Fifth Instar Silk Glands of Tasar Silkworm,Antheraea mylitta
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Analysis of Transcripts Expressed in One-Day-Old Larvae and Fifth Instar Silk Glands of Tasar Silkworm,Antheraea mylitta

机译：塔萨蚕（Antheraea mylitta）一日龄幼虫和五龄蚕丝腺中表达的转录本分析

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Antheraea mylittais one of the wild nonmulberry silkworms, which produces tasar silk. An EST project has been undertaken to understand the gene expression profile ofA. mylittasilk gland. Two cDNA libraries, one from the whole bodies of one-day-old larvae and the other from the silkglands of fifth instar larvae, were constructed and sequenced. A total of 2476 good-quality ESTs (1239 clones) were obtained and grouped into 648 clusters containing 390 contigs and 258 singletons to represent 467 potential unigenes. Forty-five sequences contained putative coding region, and represented potentially novel genes. Among the 648 clusters, 241 were categorized according to Gene Ontology hierarchy and showed presence of several silk and immune-related genes. TheA. mylittaESTs have been organized into a freely available online database “AmyBASE”. These data provide an initial insight into theA. mylittatranscriptome and help to understand the molecular mechanism of silk protein production in a Lepidopteran species.
机译：ther蚕（Antheraea mylittais）是一种野生的非桑蚕，生产塔萨尔丝。已经进行了一个EST项目以了解A的基因表达谱。蚕丝腺。构建并测序了两个cDNA文库，其中一个来自一日龄幼虫的全身，另一个来自第五龄幼虫的丝腺。总共获得了2476个高质量的EST（1239个克隆），并分为648个簇，其中包含390个重叠群和258个单例，代表了467个潜在的单基因。四十五个序列包含推定的编码区，并代表潜在的新基因。在648个簇中，有241个是根据基因本体论层次进行分类的，并显示了几个与丝绸和免疫相关的基因。 AA。 mylittaEST已被组织成一个免费的在线数据库“ AmyBASE”。这些数据提供了对A的初步了解。 mylittatranscriptome，并有助于了解鳞翅目物种中丝蛋白生产的分子机制。

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