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首页> 外文期刊>Comparative and functional genomics >Proteomic Analysis of Larval Midgut from the Silkworm (Bombyx mori)
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Proteomic Analysis of Larval Midgut from the Silkworm (Bombyx mori)

机译：家蚕幼虫中肠的蛋白质组学分析

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The midgut is the major organ for food digestion, nutrient absorption and also a barrier for foreign substance. The 5th-instar larval stage of silkworm is very important for larval growth, development, and silk production. In the present study, we used 2-DE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to analyze the midgut proteins from the 5th-instar larvae as well as the midgut proteins under starvation condition. A total of 96 proteins were identified in this study; and among them, 69 proteins were observed in midgut for the first time. We also found that the silkworm larval midgut responded to starvation by producing a 10 kDa heat shock protein and a diapause hormone precursor.
机译：中肠是食物消化，营养吸收的主要器官，也是异物的屏障。蚕的五龄幼虫期对于幼虫的生长，发育和丝绸生产非常重要。在本研究中，我们使用2-DE和基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）分析了5龄幼虫的中肠蛋白以及饥饿状况。这项研究共鉴定出96种蛋白质。其中首次在中肠观察到69种蛋白质。我们还发现，家蚕幼虫中肠通过产生10 kDa的热激蛋白和滞育激素前体来对饥饿作出反应。

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