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首页> 外文期刊>Comparative and functional genomics >Differential Protein Expression in Sugarcane during Sugarcane-Sporisorium scitamineumInteraction Revealed by 2-DE and MALDI-TOF-TOF/MS
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Differential Protein Expression in Sugarcane during Sugarcane-Sporisorium scitamineumInteraction Revealed by 2-DE and MALDI-TOF-TOF/MS

机译：2-DE和MALDI-TOF-TOF / MS揭示甘蔗-Sporisorium scitamineum相互作用期间甘蔗中的差异蛋白表达

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摘要

To understand the molecular basis of a specific plant-pathogen interaction, it is important to identify plant proteins that respond to the pathogen attack. Two sugarcane varieties, NCo376 and Ya71-374, were used in this study. By applying 2-dimensional electrophoresis (2-DE), the protein expression profile of sugarcane after inoculating withSporisorium scitamineumwas analyzed. In total, 23 differentially expressed proteins were identified by MALDI-TOF-TOF/MS. Bioinformatics analysis revealed that the functions of these 20 differential proteins were associated with such functions as photosynthesis, signal transduction, and disease resistance, while the function of the remaining three proteins was not determined. From above, we can assume that the protein regulatory network during the interaction between sugarcane andS. scitamineumis complicated. This represents the first proteomic investigation focused on highlighting the alterations of the protein expression profile in sugarcane exposed toS. scitamineum, and it provides reference information on sugarcane response toS. scitamineumstress at the protein level.
机译：要了解特定植物与病原体相互作用的分子基础，重要的是确定对病原体侵袭有反应的植物蛋白。这项研究使用了两个甘蔗品种，NCo376和Ya71-374。通过二维电泳（2-DE），分析了葡萄球菌Scitisorium scitamineum接种后甘蔗的蛋白质表达谱。总共，通过MALDI-TOF-TOF / MS鉴定了23种差异表达的蛋白质。生物信息学分析表明，这20种差异蛋白的功能与光合作用，信号转导和抗病性等功能有关，而其余三种蛋白的功能尚未确定。从上面，我们可以假设在甘蔗和S之间的相互作用中的蛋白质调控网络。 scitamineumis复杂。这代表着第一次蛋白质组学研究，重点研究了暴露于S的甘蔗中蛋白质表达谱的变化。 Scitamineum，并提供有关甘蔗对S的反应的参考信息。在蛋白质水平上的神经鞘紧张症。

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