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Ultrasound Diagnosis of Mouse Pregnancy and Gestational Staging

机译:小鼠妊娠和妊娠分期的超声诊断

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Phenotypicanalysisofmutantmiceislimitedbylackofaccurate,simple,andnondestructiveinuteroimagingtechniques.Thisstudyevaluatedtheusefulnessofultrasoundimaging(US)tostagefetalmousegestationalage(GA)anddepictmorphologicdevelopment.Weimaged16pregnantCD-1miceandatotalof92fetuseswitha15-MHzUStransducerfrom9.5dpostcoitus(DPC)until20.5DPCordelivery.Parametersrecordedincludedgestationalsacdimensions,crown#8211;rumplength(CRL),biparietaldiameter(BPD),thoracoabdominaldiameter(TAD),onsetofcardiacactivity,andmorphologicdevelopment.At9.5dDPC,allgestationsappearedasroundedsacs,withadiameter(mean±standarderror)of4.4±1mm.BPD,CRL,andGAwerehighlycorrelated.ThefollowingstructureswerefirstidentifiableatthefollowingGA:cardiacactivity,10.5DPC;majorcardiovascularstructures,11.5DPC;limbbuds,10.5DPC;spine,12.5DPC;faceandskullossification,13.5DPC;ribossification,15.5DPC;hind-andforelimbdigits,15.5DPC;stomachandurinarybladder,17.5DPC;visualizationoftherhombencephalicvesicle,13.5DPC;andvisualizationofthelateralventricles,14.5DPC.Theechogeniclungsweredistinctfromtheliverasearlyas12.5DPC.ThecircleofWilliswasdetectablewithcolorDopplerasearlyas13.5DPCandwaseasilyvisualizedat15.5DPC.WefoundthatUSprovidesaccurate,simplestagingcriteriaforfetalmousegestationaldevelopmentafter9.5DPCandmaybeanondestructivemeansofdocumentingphenotypicalterationsinmutantmiceinutero.
机译:Phenotypicanalysisofmutantmiceislimitedbylackofaccurate,简单,andnondestructiveinuteroimagingtechniques.Thisstudyevaluatedtheusefulnessofultrasoundimaging(美国)tostagefetalmousegestationalage(GA)anddepictmorphologicdevelopment.Weimaged16pregnantCD-1miceandatotalof92fetuseswitha15-MHzUStransducerfrom9.5dpostcoitus(DPC)until20.5DPCordelivery.Parametersrecordedincludedgestationalsacdimensions,冠#8211; rumplength(CRL),biparietaldiameter(BPD),thoracoabdominaldiameter(TAD)在dDP为9.5dDPC时,妊娠出现为圆形囊,直径(平均值±标准误差)为4.4±1mm.BPD,CRL和GA与高度相关。在以下情况下,可初步确定以下结构:GA,心电图,10.5DPC,主要是心血管活动,10.5DPC;主要是10.5DPC;主要是10.5DPC; ;面部和头骨脱脂,13.5DPC;骨化,15.5DPC;后肢和前肢,15.5DPC;泌尿泌尿系统膀胱,17.5DPC;本脑小泡,13.5DPC的可视化,以及旁通者的可视化第14.5 DPC条,从肝脏中吸收的致食性肺炎的可能性约为12.5 DPC。在彩色多普勒酶中可见13.5 DPC可以检测到威利斯环,并在15.5 DPC处易于观察到。我们发现,在9.5点之后,美国提供了准确的,简化的,可简化的计算机化的标准,以期对胎儿的吞噬进行了详尽的描述,并为以后的研究提供了可能。

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