Feasibility and Limits of an Orthotopic Human Colon Cancer Model in Nude Mice

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Feasibility and Limits of an Orthotopic Human Colon Cancer Model in Nude Mice

机译：裸鼠原位人类结肠癌模型的可行性和局限性

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Wesoughttodevelopanaccuratecolorectalcancermodelinnudemicewithstablelocalgrowth,tumorcelldissemination,andreproduciblemetastaticcapacity.Tothisend,weorthotopicallytransplantedhistologicallyintacthumancolorectalcancertissuefrom10humanpatientsintonudemice.Aftersuccessfullocaltumorgrowth,tumortissueswereretransplantedasmanyas9timesinserialpassage.Allspecimensweretransplantedusingmicrosurgicaltechniques.Histologic,immunohistochemical,andpolymerasechainreactiontechniqueswereusedtodeterminetumorgrowthratesandkinetics,developmentofregionallymphnodeanddistanthepaticmetastases,andtheinductionofminimalresidualdisease(MRD).Stablelocaltumorgrowthrateswithvariablegrowthkineticsweredetectedin73.4%ofallmice.Thelymphnodeandhepaticmetastasisrateswerelow,at18.4%and4.9%,respectively.MRD,asreflectedbyCK20positivityofthebonemarrowinanimalswithlymphnodeandhepaticmetastases,waspresentin22.2%.Theorthotopiccolorectalcancermodeldescribedhereisfeasiblefortheinductionofreproduciblelocaltumorgrowthbutislimitedbyvariablegrowthkineticsandthelowrateoflymphnodeandhepaticmetastases.Cytokeratin-positivecellsindicativeofMRDcouldbedetectedinthebonemarrowofapproximately25%ofthenudemicewithmetastases.TheobservedinductionofMRDafterorthotopicimplantationofintacthumancoloncancerinanimalswithlymphnodeandhepaticmetastasesmightbeimprovedifestablishedcoloncancercelllineswereused.

机译：Wesoughttodevelopanaccuratecolorectalcancermodelinnudemicewithstablelocalgrowth，tumorcelldissemination，andreproduciblemetastaticcapacity.Tothisend，weorthotopicallytransplantedhistologicallyintacthumancolorectalcancertissuefrom10humanpatientsintonudemice.Aftersuccessfullocaltumorgrowth，tumortissueswereretransplantedasmanyas9timesinserialpassage.Allspecimensweretransplantedusingmicrosurgicaltechniques.Histologic，免疫组织化学，andpolymerasechainreactiontechniqueswereusedtodeterminetumorgrowthratesandkinetics，developmentofregionallymphnodeanddistanthepaticmetastases，andtheinductionofminimalresidualdisease（MRD）.Stablelocaltumorgrowthrateswithvariablegrowthkineticsweredetectedin73.4％ofallmice.Thelymphnodeandhepaticmetastasisrateswerelow，at18.4％and4.9％，respectively.MRD，由淋巴结和肝转移的骨髓动物的CK20阳性所反映的阳性率为22.2％。此处描述的原位结直肠癌模型可诱导可再生的局部肿瘤生长，但受血管的限制可能的生长动力学和较低的淋巴结转移率和肝转移率。可以在大约25％的转移率的骨髓中检测到指示MRD的细胞角蛋白阳性细胞。

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《Comparative Medicine》 |2006年第2期|共页
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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1. Feasibility and limits of an orthotopic human colon cancer model in nude mice [J] . Thalheimer A, Illert B, Bueter M, Comparative Medicine . 2006,第2期

机译：原位人结肠癌裸鼠模型的可行性和局限性
2. Metastatic Model of Human Colon Cancer Constructed using Orthotopic Implantation in Nude Mice [J] . YUTAKA OGATA, YASUSHI HARA, YOSHITO AKAGI, Kurume Medical Journal . 1998,第1期

机译：原位植入裸鼠建立人结肠癌转移模型
3. Tumor-sealing Surgical Orthotopic Implantation of Human Colon Cancer in Nude Mice Induces Clinically-relevant Metastases Without Early Peritoneal Carcinomatosis [J] . Yoon Sang Nam, Park Jun Ho, Lwin Thinzar M., Anticancer Research: International Journal of Cancer Research and Treatment . 2019,第8期

机译：在裸鼠中肿瘤密封手术原位植入人结肠癌诱导临床相关转移，没有早期腹膜癌症
4. CDNA MICRO ARRAY ANALYSIS FOR TRANSPLANTED CANINE OSTEOSARCOMA CELLS INTO THE ORTHOTOPIC OR ECTOPIC SITE IN A NUDE MICE MODEL [C] . Takayuki Nakagawa, Takehiko Inagaki, Yoshifumi Endo, Annual conference of the Veterinary Cancer Society . 2008

机译：将移植犬骨肉瘤细胞移植到裸鼠模型中的原位或异位位点的cDNA微阵列分析
5. Cancer cachexia and cardiac atrophy in the APCMin/+ mice model of colon cancer. [D] . Manne, Nandini Durga Prasanna Kumar. 2011

机译：结肠癌APCMin / +小鼠模型中的癌症恶病质和心脏萎缩。
6. Models of human metastatic colon cancer in nude mice orthotopically constructed by using histologically intact patient specimens. [O] . X Y Fu, J M Besterman, A Monosov, 1991

机译：通过使用组织学完整的患者标本原位构建裸鼠中的人类转移性结肠癌模型。
7. Models of human metastatic colon cancer in nude mice orthotopically constructed by using histologically intact patient specimens. [O] . Fu, X Y, Besterman, J M, Monosov, A, 1991

机译：通过使用组织学完整的患者标本原位构建裸鼠中的人类转移性结肠癌模型。

Feasibility and Limits of an Orthotopic Human Colon Cancer Model in Nude Mice

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