Development and Use of a Simple Polymerase Chain Reaction Assay to Screen for Helicobacter spp. and H. hepaticus in Intestinal and Fecal Samples from Laboratory Mice

Fermér Christian; Lindberg Anna V.; Feinstein Ricardo E.

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Development and Use of a Simple Polymerase Chain Reaction Assay to Screen for Helicobacter spp. and H. hepaticus in Intestinal and Fecal Samples from Laboratory Mice

机译：筛选幽门螺杆菌的简单聚合酶链反应分析方法的开发和应用。和H. hepaticus在肠道和粪便样本中的实验小鼠

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Asimpleandsensitiveduplexpolymerasechainreaction(PCR)assaywasdevelopedforuseindetectionofHelicobacterspeciesandH.hepaticusinlaboratorymice.Bacteriawereextractedandconcentratedfromfecalpelletsandintestinalsegmentsbyuseofbuoyantdensitycentrifugation.Toimprovequalityassurance,aninternalcontrol(mimic)fordetectionoffalse-negativereactionswasincluded.Inaddition,cartridges(Capillette)pre-filledwithPCRreagents,wereusedtominimizethehands-ontimerequired,thusreducingtheriskofcontaminationwithpreviouslyamplifiedmaterial.LaboratorymicefromSwedishanimalhousessenttotheNationalVeterinaryInstituteforhealthmonitoringwerefoundtohavehighprevalenceofH.hepaticus.

机译：Asimpleandsensitiveduplexpolymerasechainreaction（PCR）assaywasdevelopedforuseindetectionofHelicobacterspeciesandH.hepaticusinlaboratorymice.Bacteriawereextractedandconcentratedfromfecalpelletsandintestinalsegmentsbyuseofbuoyantdensitycentrifugation.Toimprovequalityassurance，aninternalcontrol（模拟物）fordetectionoffalse-negativereactionswasincluded.Inaddition，药筒（Capillette）预filledwithPCRreagents，wereusedtominimizethehands-ontimerequired，thusreducingtheriskofcontaminationwithpreviouslyamplifiedmaterial.LaboratorymicefromSwedishanimalhousessenttotheNationalVeterinaryInstituteforhealthmonitoringwerefoundtohavehighprevalenceofH.hepaticus。

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《Comparative Medicine》 |2002年第6期|共页
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Fermér Christian; Lindberg Anna V.; Feinstein Ricardo E.;
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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1. Development and use of a simple polymerase chain reaction assay to screen for Helicobacter spp. and H-hepaticus in intestinal and fecal samples from laboratory mice [J] . Fermer C, Lindberg AV, Feinstein RE Comparative Medicine . 2002,第6期

机译：开发和使用简单的聚合酶链反应分析法筛选幽门螺杆菌。小鼠肠道和粪便样品中的H和肝肝
2. Monitoring Sentinel Mice for Helicobacter hepaticus, H. rodentium, and H. bilis Infection by Use of Polymerase Chain Reaction Analysis and Serologic Testing [J] . Comparative Medicine . 2000,第4期

机译：通过聚合酶链反应分析和血清学检测监测前哨鼠肝炎，啮齿类杆菌和胆汁感染
3. Evaluation of a Commercial Colorimetric Fecal Dipstick Assay for the Detection of Helicobacter hepaticus Infections in Laboratory Mice [J] . Freebersyser Julie E., Drake Michael T., Riley Lela K., Journal of the American Association for Laboratory Animal Science . 2010,第3期

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5. Development of polymerase chain reaction amplification assay as a screening test for identifying dairy herds infected with bovine viral diarrhea virus [D] . Radwan, Gamal Soliman 1994

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6. Evaluation of a Commercial Colorimetric Fecal Dipstick Assay for the Detection of Helicobacter hepaticus Infections in Laboratory Mice [O] . Julie E Freebersyser, Michael T Drake, Lela K Riley, 1997

机译：商业比色粪便试纸法检测实验室小鼠肝炎性感染的评估。
7. Rapid detection of avian influenza virus in chicken fecal samples by immunomagnetic capture reverse transcriptase–polymerase chain reaction assay [O] . Dhumpa Raghuram, Handberg Kurt, Jørgensen Poul Henrik, 2011

机译：通过免疫磁性捕获逆转录酶 - 聚合酶链反应快速检测鸡粪样品中的禽流感病毒
8. Effects of Holding Time, Storage, and the Preservation of Samples on Sample Integrity for the Detection of Fecal Indicator Bacteria by Quantitative Polymerase Chain Reaction (QPCR)-based Assays. [R] . Wymer, L., Oshima, K., Paar, J., 2010

机译：保留时间，储存和样品保存对样品完整性的影响，用于基于定量聚合酶链反应（QpCR）的分析检测粪便指示菌。

Development and Use of a Simple Polymerase Chain Reaction Assay to Screen for Helicobacter spp. and H. hepaticus in Intestinal and Fecal Samples from Laboratory Mice

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