Isolation, Transplantation, and Functional Studies of Adult Porcine Islets of Langerhans

Nielsen; Thomas Buschmann; nbsp; Yderstraede; Knud Bonnet; nbsp; Beck-Nielsen; Henning

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Isolation, Transplantation, and Functional Studies of Adult Porcine Islets of Langerhans

机译：朗格汉斯成人猪胰岛的分离，移植和功能研究

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TransplantationofisletsofLangerhansisapossibletreatmentfortype-Idiabetesmellitus.However,thereisashortageofdonorsforsuchtransplantationsandthepigmaybeanalternativesourceofdonororgans.TheaimsofthestudyreportedhereweretoestablishamethodforadultporcineisletisolationthatwasbasedonenzymaticdigestionusingLiberasePIinasemiautomaticset-up,andtoevaluatetheinvitroandinvivofunctionofisolatedislets.Afterovernightculture,isolatedislets,fromfiveofsevenbatches,hadpoorinsulinresponsetoaninvitroglucosechallengethatwasonlypartiallyincreasedbyadditionalchallengewitharginine.Morethan50%ofDNAand90%oftheinsulincontentwaslostduringaone-weekcultureperiod.Withsomebatch-to-batchvariation,in15of25cases,4,000to7,000porcineisletscuredstreptozotocindiabeticnudemicewithinthreeweeksfollowingtransplantation.brxmlns="http://pub2web.metastore.ingenta.coms/"/brInconclusion,itispossibletoisolateviableisletsfromadultpigs,usingasemiautomaticset-up.Withbatch-to-batchvariation,theisletsareabletorevertdiabetesmellituswhentransplantedtodiabeticnudemice.

机译：TransplantationofisletsofLangerhansisapossibletreatmentfortype-Idiabetesmellitus.However，thereisashortageofdonorsforsuchtransplantationsandthepigmaybeanalternativesourceofdonororgans.TheaimsofthestudyreportedhereweretoestablishamethodforadultporcineisletisolationthatwasbasedonenzymaticdigestionusingLiberasePIinasemiautomaticset镜头，andtoevaluatetheinvitroandinvivofunctionofisolatedislets.Afterovernightculture，isolatedislets，fromfiveofsevenbatches，hadpoorinsulinresponsetoaninvitroglucosechallengethatwasonlypartiallyincreasedbyadditionalchallengewitharginine.Morethan50％ofDNAand90％oftheinsulincontentwaslostduringaone-weekcultureperiod.Withsomebatch到batchvariation，in15of25cases，4,000to7,000porcineisletscuredstreptozotocindiabeticnudemicewithinthreeweeksfollowingtransplantation 结论是，有可能使用半自动设置方法从成年猪中分离出可行的胰岛。随着批次间的变化，这些胰岛可用于恢复糖尿病。 n移植至糖尿病性尿毒症。

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《Comparative Medicine》 |2002年第2期|共页
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Nielsen; Thomas Buschmann; nbsp; Yderstraede; Knud Bonnet; nbsp; Beck-Nielsen; Henning;
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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1. Cost and Scalability Analysis of Porcine Islet Isolation for Islet Transplantation: Comparison of Juvenile, Neonatal and Adult Pigs [J] . Vanderschelden Rachel, Sathialingam Mayilone, Alexander Michael, Cell transplantation . 2019,第7期

机译：胰岛移植猪胰岛分离的成本和可扩展性分析：青少年，新生儿和成人猪的比较
2. Isolation, density purification, and in vitro culture maintenance of functional caprine islets of Langerhans as an alternative islet source for diabetes study. [J] . Hani H, Ibrahim TA, Othman AM, Xenotransplantation . 2010,第6期

机译：朗格汉斯功能山羊胰岛的分离，密度纯化和体外培养维持，作为糖尿病研究的替代胰岛来源。
3. Isolation outcome and functional characteristics of young and adult pig pancreatic islets for transplantation studies. [J] . Bottino R, Balamurugan AN, Smetanka C, Xenotransplantation . 2007,第1期

机译：青年和成年猪胰岛的分离结果和功能特性，用于移植研究。
4. Quadrupole Magnetic Separation (Qms) of Porcine Islets of Langerhans [C] . Jeffrey Chalmers, Klearchos Papas, Rustin Shenkman International Symposium on Runaway Reactions, Pressure Relief Design and Effluent Handling American Institute of Chemical Engineers/American Chemical Society Management Conference . 2005

机译：朗格汉兰猪胰岛磁性分离（QMS）
5. Factors influencing the early results of pancreatic islet transplantation: Studies on isolation enzymes and islet revascularisation - Summary. [D] . Mathe, Zoltan. 2007

机译：影响胰岛移植早期结果的因素：分离酶和胰岛血运重建的研究-摘要。
6. Cost and Scalability Analysis of Porcine Islet Isolation for Islet Transplantation: Comparison of Juvenile Neonatal and Adult Pigs [O] . Rachel Vanderschelden, Mayilone Sathialingam, Michael Alexander, 2019

机译：猪胰岛移植的成本和可扩展性分析：幼猪新生猪和成年猪的比较
7. Transplante isogênico de ilhotas de Langerhans no fígado de ratos: metodologia para separação e purificação das ilhotas de Langerhans Isogenic islet transplantation on the rat liver: Method for isolation and purification of the rat islets [O] . Eleazar CHAIB, Apostolos PAPALOIS, Ingrid G. M. BRONS, 2000

机译：朗格汉斯胰岛在大鼠肝脏中的同基因移植：朗格汉斯胰岛同种异体胰岛移植胰岛的分离纯化方法

Isolation, Transplantation, and Functional Studies of Adult Porcine Islets of Langerhans

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