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首页> 外文期刊>Biology Open >Location and functions of Inebriated in the Drosophila eye
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Location and functions of Inebriated in the Drosophila eye

机译：果蝇眼中醉酒的位置和功能

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Histamine (HA) is a neurotransmitter in arthropod photoreceptors. It is recycled via conjugation to β-alanine to form β-alanylhistamine (carcinine). Conjugation occurs in epithelial glia that surround photoreceptor terminals in the first optic neuropil, and carcinine (CA) is then transported back to photoreceptors and cleaved to liberate HA and β-alanine. The geneInebriated(Ine) encodes an Na+/Cl?-dependent SLC6 family transporter translated as two protein isoforms, long (P1) and short (P2). Photoreceptors specifically express Ine-P2 whereas Ine-P1 is expressed in non-neuronal cells. Bothine1andine3have significantly reduced head HA contents compared with wild type, and a smaller increase in head HA after drinking 1% CA. Similarly, uptake of 0.1% CA was reduced inine1andine3mutant synaptosomes, but increased by 90% and 84% respectively for fractions incubated in 0.05% β-Ala, compared with wild type. Screening potential substrates in Ine expressingXenopusoocytes revealed very little response to carcinine and β-Ala but increased conductance with glycine. Bothine1andine3mutant responses in light-dark phototaxis did not differ from wild-type. Collectively our results suggest that Inebriated functions in an adjunct role as a transporter to the previously reported carcinine transporter CarT.
机译：组胺（HA）是节肢动物感光器中的神经递质。通过与β-丙氨酸结合而再循环以形成β-丙氨酰组胺（carcinine）。共轭发生在第一个视神经细胞中的感光细胞末端周围的上皮神经胶质中，然后将癌素（CA）转运回感光细胞，并裂解以释放HA和β-丙氨酸。 GeneInebriated（Ine）编码一个依赖Na + / Cl2的SLC6家族转运蛋白，翻译成两种蛋白质同种型，长（P1）和短（P2）。感光细胞特异性表达Ine-P2，而Ine-P1在非神经元细胞中表达。与野生型相比，bineine1和ine3均显着降低了头部HA含量，饮用1％CA后，头部HA的增加幅度较小。类似地，与野生型相比，在inine1和ine3突变突触体中摄取0.1％CA会减少，但在0.05％β-Ala中孵育的级分分别增加90％和84％。筛选表达Ine的异型异种细胞中潜在的底物，发现对癌素和β-Ala的反应极少，但甘氨酸的电导增加。浅色趋光性中的bothine1和ine3突变响应与野生型无差异。总体而言，我们的研究结果表明，Inbrried在以前报道的癌性转运蛋白CarT的转运蛋白中起辅助作用。

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