• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical & developmental immunology. >The Subcellular Localization of the Receptor for Platelet-Activating Factor in Neutrophils Affects Signaling and Activation Characteristics
【24h】

The Subcellular Localization of the Receptor for Platelet-Activating Factor in Neutrophils Affects Signaling and Activation Characteristics

机译：中性粒细胞中血小板活化因子受体的亚细胞定位影响信号传导和活化特性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The localization in neutrophils, of the receptor for platelet-activating factor (PAFR), has been determined using subcellular fractionation and a receptor mobilization protocol. We show that the PAFR is expressed primarily in the plasma membrane. Although activation of neutrophils by PAF induces responses typical also of agonists that bind the formyl peptide receptors (FPR), known to be stored in mobilizable organelles, some quantitative as well as qualitative differences were observed when neutrophils were activated through these receptors. PAF is equipotent to fMLF (high affinity agonist for FPR1) to cleave off L-selectin and to induce granule/vesicle secretion but is more potent than fMLF to induce a rise in intracellular Ca~(2+). Similar to fMLF, PAF induced also a robust release of reactive oxygen species, but with higher EC_(50)value and was less sensitive to a PI3K inhibitor compared to the fMLF response. Despite the lack of a granule localized storage pool of receptors, the PAF-induced superoxide production could be primed; receptor mobilization was, thus, not required for priming of the PAF response. The desensitized PAFR could not be reactivated, suggesting that distinct signaling pathways are utilized for termination of the responses triggered through FPR1 and PAFR.
机译：血小板激活因子（PAFR）受体在嗜中性粒细胞中的定位已使用亚细胞分级分离和受体动员方案确定。我们表明，PAFR主要在质膜中表达。尽管通过PAF激活嗜中性粒细胞会诱发典型的结合甲酰肽受体（FPR）的激动剂的反应，已知激动剂会储存在可动的细胞器中，但是当通过这些受体激活嗜中性粒细胞时，观察到一些定量和质量上的差异。 PAF与fMLF（FPR1的高亲和力激动剂）具有等价作用，可切割L-选择素并诱导颗粒/囊泡分泌，但比fMLF诱导细胞内Ca〜（2+）升高的能力更强。类似于fMLF反应，PAF还诱导了活性氧的强烈释放，但具有较高的EC_（50）值，并且对PI3K抑制剂的敏感性低于fMLF反应。尽管缺乏受体的颗粒状局部贮存池，但PAF诱导的超氧化物产生仍可被引发。因此，启动PAF反应不需要受体动员。脱敏的PAFR不能重新激活，表明利用了不同的信号通路来终止FPR1和PAFR触发的应答。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号